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Catalog Number	Size	Price
LS-C675302-50	50 µl	$316
LS-C675302-100	100 µl (0.33 mg/ml)	$386

CD99 Antibody - Immunohistochemistry Dilution at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.

CD99 Antibody - Western Blot Positive WB detected in:Jurkat whole cell lysate All Lanes:CD99 antibody at 0.8µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 28 KDa Observed band size: 28 KDa

CD99 Antibody - Overlay histogram showing Jurkat cells stained (red line) at dilution of 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal Goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C.The Secondary antibody used was FITC Goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

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Monoclonal Rabbit anti‑Human CD99 Antibody (IHC, WB) LS‑C675302

Name: Anti-CD99 Antibody | Rabbit anti-Human Monoclonal ELISA,Flo,IHC,WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C675302-50
Price: 316.0000 USD
Availability: InStock

Submit Review LSBio Guarantee

Antibody:

CD99 Rabbit anti-Human Monoclonal Antibody

Application:

IHC, IHC-P, WB, Flo, ELISA

Reactivity:

Human

Format:

Unconjugated, Unmodified

$316

LS-C675302-50

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

CD99 Rabbit anti-Human Monoclonal Antibody

Application:

IHC, IHC-P, WB, Flo, ELISA

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

CD99 antibody LS-C675302 is an unconjugated rabbit monoclonal antibody to human CD99. Validated for ELISA, Flow, IHC and WB.

Target

Human CD99

Synonyms

See All CD99 Antibodies

Host

Rabbit

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

IgG Monoclonal

Conjugations

Unconjugated

Purification

Affinity chromatography

Modifications

Unmodified

Immunogen

A synthesized peptide

Applications

IHC
IHC - Paraffin
Western blot
Flow Cytometry
ELISA

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

PBS, pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About CD99

Uniprot: P14209 NCBI: NM_002414 NP_002405.1