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Antibodies > CD81 > LS-C675300
 
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Catalog Number Size Price
LS-C675300-50 50 µl (0.8 mg/ml) $316 
LS-C675300-100 100 µl (1 mg/ml) $386 
CD81 Antibody - Immunohistochemistry Dilution at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
CD81 Antibody - Immunohistochemistry Dilution at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
CD81 Antibody - Western Blot Positive WB detected in:K562 whole cell lysate All Lanes:CD81 antibody at 1.25µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 26 KDa Observed band size: 26 KDa
CD81 Antibody - Overlay histogram showing Jurkat cells stained with CD81 Antibody (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4?.The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4?. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
CD81 Antibody - Immunohistochemistry Dilution at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
CD81 Antibody - Immunohistochemistry Dilution at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
CD81 Antibody - Western Blot Positive WB detected in:K562 whole cell lysate All Lanes:CD81 antibody at 1.25µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 26 KDa Observed band size: 26 KDa
CD81 Antibody - Overlay histogram showing Jurkat cells stained with CD81 Antibody (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4?.The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4?. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
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Monoclonal Rabbit anti‑Human CD81 Antibody (IHC, WB) LS‑C675300

Monoclonal Rabbit anti‑Human CD81 Antibody (IHC, WB) LS‑C675300

Submit Review LSBio Guarantee
Antibody:
CD81 Rabbit anti-Human Monoclonal Antibody
Application:
IHC, IHC-P, WB, Flo, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$316
LS-C675300-50
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
CD81 Rabbit anti-Human Monoclonal Antibody
Application:
IHC, IHC-P, WB, Flo, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
CD81 antibody LS-C675300 is an unconjugated rabbit monoclonal antibody to CD81 from human. It is reactive with human, mouse and rat. Validated for ELISA, Flow, IHC and WB.
Target
Human CD81
Synonyms
CD81 | CD81 antigen | CD81 molecule | CVID6 | S5.7 | TAPA1 | TAPA-1 | Tetraspanin-28 | TSPAN28 | Tspan-28
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Monoclonal
Conjugations
Unconjugated
Purification
Affinity chromatography
Modifications
Unmodified
Immunogen
A synthesized peptide
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
  • Flow Cytometry
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CD81
P60033 NM_004356 NP_004347.1

Publications (0)

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Customer Reviews (0)

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CD81 Antibody - Flow cytometry of CD81 antibody This image was taken for the unconjugated form of this product. Other forms have not been tested.
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IHC‑plus™ CD81 Antibody LS‑B6373
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See all CD81 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/18/2024
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