Mouse CD81

Hamster

Mouse (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

Eat2

Unconjugated. Also available conjugated with RPE, FITC.

Protein G purified

Unmodified

38C13, murine B cell line.

Recognizes mouse and rat CD81, also known as TAPA-1 or Target of the antiproliferative antibody 1. CD81 is a 236 amino acid ~26 kD multipass transmembrane protein belonging to the TM4SF family. In rodents CD81 is expressed at much higher levels on resting B cells than on T cells, although increased expression on T cells is found following activation. Induces homotypic aggregation of B cells and inhibits anti Ig and IL-4 induced proliferation. Eat 2 requires the presence of both extracellular loops of TAPA-1 for binding. Mice lacking CD81 demonstrate reduced fertility through impaired oocyte-sperm fusion, double knockout CD81-/- CD9-/- mice are completely infertile suggesting complementary roles in oocyte-sperm fusion.

• IHC - Frozen
• Western blot
• Immunoprecipitation
• Flow Cytometry (1:10 - 1:50)
• ELISA

Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul.

PBS, 0.09% Sodium Azide

Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P60033 NCBI: NM_004356 NP_004347.1