Human CD58

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

Biotin

Protein A purified

Unmodified

Human cytotoxic T cells.

Reacts with both transmembrane and GPI anchored CD58. Recognizes the CD58 glycoprotein of about 60-70kD and will inhibit HLA-DR mediated T cell cytotoxicity.

• IHC
• IHC - Frozen
• Immunoprecipitation
• Flow Cytometry
• Cytotoxic Assay

Antibody will inhibit HLA-DR mediated T cell cytotoxicity. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation. Immunohistochemistry: Frozen. Flow Cytometry: 5x10^5 cultured human Nalm-6 cells were washed and incubated 45 minutes on ice with 80 ml of anti-CD58/Biotin at 10 mg/ml. Cells were washed twice and incubated with 2? reagent Streptavidin/R-PE after which they were washed three times, fixed and analyzed using a BD FACS Calibur. Cells stained positive with a mean shift of 1.29 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration. Binding was 94% blocked when pre incubated 10 minutes with 20 ml of 0.5 mg/ml anti-CD58 antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

PBS, pH 7.2, 0.05% Sodium Azide, 5% Glycerol, 0.2% BSA

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P19256 NCBI: NM_001779 NP_001770.1