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Catalog Number	Size	Price
LS-C756407-10	10 µg	$318
LS-C756407-100	100 µg	$470

CD5 Antibody - IHC analysis of CD5 using anti-CD5 antibody. CD5 was detected in paraffin-embedded section of human tonsil tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2?g/ml rabbit anti-CD5 Antibody overnight at 4?C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

CD5 Antibody - IHC analysis of CD5 using anti-CD5 antibody. CD5 was detected in paraffin-embedded section of mouse spleen tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2?g/ml rabbit anti-CD5 Antibody overnight at 4?C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

CD5 Antibody - IHC analysis of CD5 using anti-CD5 antibody. CD5 was detected in paraffin-embedded section of rat spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml rabbit anti-CD5 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

CD5 Antibody - IF analysis of CD68 and CD5 using anti-CD68 antibody (M00602) and anti-CD5 antibody CD68 and CD5 was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/mL rabbit anti-CD68 antibody (M00602) and anti-CD5 antibody overnight at 4°C. DyLight488 Conjugated Goat Anti-Mouse IgG and Biotin conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

CD5 Antibody - Western blot analysis of CD5 using anti-CD5 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates,Lane 2: human CCRF-CEM whole cell lysates,Lane 3: mouse NIH3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD5 antigen affinity purified polyclonal antibody at 0.5 ?g/mL overnight at 4?C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD5 at approximately 67KD. The expected band size for CD5 is at 54KD.

CD5 Antibody - Flow Cytometry analysis of EL-4 cells using anti-CD5 antibody. Overlay histogram showing EL-4 cells stained with anti-CD5 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD5 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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Polyclonal Rabbit anti‑Human CD5 Antibody (IHC, WB) LS‑C756407

Name: Anti-CD5 Antibody | Rabbit anti-Human Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C756407-10
Price: 318.0000 USD
Availability: InStock

Submit Review LSBio Guarantee

Antibody:

CD5 Rabbit anti-Human Polyclonal Antibody

Application:

IHC-P, IHC-Fr, ICC, WB, Flo, ELISA

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

$318

LS-C756407-10

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

CD5 Rabbit anti-Human Polyclonal Antibody

Application:

IHC-P, IHC-Fr, ICC, WB, Flo, ELISA

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

Specifications

Description

CD5 antibody LS-C756407 is an unconjugated rabbit polyclonal antibody to CD5 from human. It is reactive with human, mouse and rat. Validated for ELISA, Flow, ICC, IHC and WB.

Target

Human CD5

Synonyms

CD5 | CD5 antigen | CD5 antigen (p56-62) | CD5 molecule | Lymphocyte antigen T1/Leu-1 | LEU1 | T1

See All CD5 Antibodies

Host

Rabbit

Reactivity

Human, Mouse, Rat (tested or 100% immunogen sequence identity)

Clonality

IgG Polyclonal

Conjugations

Unconjugated

Purification

Immunogen affinity purified

Modifications

Unmodified

Immunogen

E. coli-derived human CD5 recombinant protein (Position: R25-L495).

Specificity

No cross reactivity with other proteins.

Applications

IHC - Paraffin (0.5 - 1 µg/ml)
IHC - Frozen (0.5 - 1 µg/ml)
ICC (0.5 - 1 µg/ml)
Western blot (0.1 - 0.5 µg/ml)
Flow Cytometry (1 - 3 µg/10E6 cells)
ELISA (0.1 - 0.5 µg/ml)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

Applications should be user optimized.

Presentation

Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Storage

After reconstitution, may be stored at 4°C for 1 month. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.