Human CD5

Mouse

Human, Rhesus monkey (tested or 100% immunogen sequence identity)

IgG2a,k Monoclonal

CRIS-1

Unconjugated

Protein G purified

Azide-free, BSA-free. Also available Unmodified.

Stimulated human leukocytes were used as the immunogen for this CD5 antibody.

Human CD5

• IHC
• IHC - Frozen
• Immunofluorescence (0.5 - 1 µg/ml)
• Immunoprecipitation
• Flow Cytometry (0.5 - 1 µg/10E6 cells)
• ELISA
• Functional Assay

The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. This antibody recognizes a 67kDa transmembrane protein, which is identified as CD5. It is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in Tcell areas. CD5 antibody is a pan Tcell marker that also reacts with a range of neoplastic Bcells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma, and a subset (~10%) of diffuse large Bcell lymphoma. Aberrant expression is useful in making a diagnosis of mature Tcell neoplasms. CD5 antibody detection is diagnostic in CLL/SLL within a panel of other Bcell markers, especially one that includes anti-CD23. CD5 antibody is also very useful in differentiating among mature small lymphoid cell malignancies. In addition, it can be used in distinguishing thymic carcinoma (+) from thymoma (-). It does not react with granulocytes or monocytes. ELISA: Order BSA-free format for coating.

1X PBS

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P06127 NCBI: NM_014207 NP_055022.2