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Catalog Number	Size	Price
LS-C357794-20	20 µg (0.2 mg/ml)	$371
LS-C357794-100	100 µg (0.2 mg/ml)	$574

CD47 Antibody - Western blot testing of human brain lysate with CD47 antibody (clone B6H12.2). Predicted molecular weight ~35kDa but observed at 35-60kDa depending on glycosylation level.

Monoclonal Mouse anti‑Human CD47 Antibody (clone B6H12.2, IHC, IF, WB) LS‑C357794

Name: Anti-CD47 Antibody | Mouse anti-Human Monoclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C357794-20
Price: 371.0000 USD
Availability: InStock

Submit Review LSBio Guarantee

Antibody:

CD47 Mouse anti-Human Monoclonal (B6H12.2) Antibody

Application:

IHC, IHC-P, IF, WB, Flo, Block, Func

Reactivity:

Human

Format:

Unconjugated, Unmodified

Other formats:

Azide-free, BSA-free

$371

LS-C357794-20

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

CD47 Mouse anti-Human Monoclonal (B6H12.2) Antibody

Application:

IHC, IHC-P, IF, WB, Flo, Block, Func

Reactivity:

Human

Format:

Unconjugated, Unmodified

Other formats:

Azide-free, BSA-free

Specifications

Description

CD47 antibody LS-C357794 is an unconjugated mouse monoclonal antibody to human CD47. Validated for Block, Flow, Func, IF, IHC and WB.

Target

Human CD47

Synonyms

See All CD47 Antibodies

Host

Mouse

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

IgG1,k Monoclonal

Clone

B6H12.2

Conjugations

Unconjugated

Purification

Protein G purified

Modifications

Unmodified. Also available Azide-free, BSA-free.

Immunogen

Intact CD47 purified from placenta.

Specificity

Human CD47

Applications

IHC
IHC - Paraffin (0.5 - 1 µg/ml)
Immunofluorescence (1 - 2 µg/ml)
Western blot
Flow Cytometry (0.5 - 1 µg/10E6 cells)
Blocking
Functional Assay

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

This antibody reacts with the Ig domain of CD47 protein. It has been shown to inhibit polymorphonuclear neutrophil (PMN) transmigration across cell monolayers and matrix. CD47, originally named integrin-associated protein (IAP), is a 50kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction. Functional Activity/blocking: Order BSA/Azide-free format

Presentation

1X PBS, 0.1 mg/ml BSA, 0.05% sodium azide

Storage

Store at 2°C to 8°C.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About CD47

Uniprot: Q08722 NCBI: NM_001777 NP_001768.1