Monoclonal Mouse anti‑Human CD47 Antibody (clone B6H12.2, Azide‑free,BSA‑free, IHC, IF, WB) LS‑C389481
Monoclonal Mouse anti‑Human CD47 Antibody (clone B6H12.2, Azide‑free,BSA‑free, IHC, IF, WB) LS‑C389481
Antibody:
CD47 Mouse anti-Human Monoclonal (Azide-free, BSA-free) (B6H12.2) Antibody
Application:
IHC, IHC-P, IF, WB, Flo, Block, Func
Format:
Unconjugated, Azide-free, BSA-free
Overview
Antibody:
CD47 Mouse anti-Human Monoclonal (Azide-free, BSA-free) (B6H12.2) Antibody
Application:
IHC, IHC-P, IF, WB, Flo, Block, Func
Format:
Unconjugated, Azide-free, BSA-free
Specifications
Description
CD47 antibody LS-C389481 is an unconjugated mouse monoclonal antibody to human CD47. Validated for Block, Flow, Func, IF, IHC and WB.
Target
Human CD47
Synonyms
CD47 | CD47 antigen | CD47 molecule | Integrin associated protein | Integrin-associated protein | Leukocyte surface antigen CD47 | MER6 | Protein MER6 | Rh-related antigen | OA3 | CD47 glycoprotein | IAP
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Azide-free, BSA-free.
Also available
Unmodified.
Immunogen
Intact CD47 purified from placenta.
Applications
- IHC
- IHC - Paraffin (0.5 - 1 µg/ml)
- Immunofluorescence (1 - 2 µg/ml)
- Western blot
- Flow Cytometry (0.5 - 1 µg/10E6 cells)
- Blocking
- Functional Assay
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Usage
The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. This antibody reacts with the Ig domain of CD47 protein. It has been shown to inhibit polymorphonuclear neutrophil (PMN) transmigration across cell monolayers and matrix. CD47, originally named integrin-associated protein (IAP), is a 50kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction. Functional Activity/blocking: Order BSA/Azide-free format
Storage
Store at 2°C to 8°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, ELISA
Species:
Human
Applications:
IHC, IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry
Species:
Mouse
Applications:
Western blot
Species:
Human
Applications:
IHC, IHC - Frozen, Immunoprecipitation, Flow Cytometry
Species:
Human
Applications:
Flow Cytometry
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/24/2024