Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Phycoerythrin.
Also available
Unconjugated or conjugated with
FITC.
Purification
Protein A/G purified
Immunogen
Peripheral blood monocytes from rheumatoid arthritis patient.
Specificity
Human PTPRC / CD45
Applications
- Flow Cytometry
- Applications tested for the base form of this product only
Usage
Add 10 ul of antibody / 100 ul of 10^6 PBMC in PBS. Mix Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. Add 10 ul of antibody / 100 ul of Whole Blood. Mix gently and incubate for 15 minutes at room temperature 20°C. Lyse Whole Blood and analyze or fix with 0.5% v/v paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, 0.08% sodium azide, 0.2% carrier protein, sterile-filtered
Storage
Store at 4°C. Do not freeze.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee