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Catalog Number Size Price
LS-C747519-20 20 µl $275 
LS-C747519-100 100 µl (2.32 mg/ml) $389 
CD3E Antibody - Immunohistochemistry of paraffin-embedded mouse thymus using CD3E antibody at dilution of 1:100 (40x lens).
CD3E Antibody - Immunohistochemistry of paraffin-embedded mouse spleen using CD3E antibody at dilution of 1:100 (40x lens).
CD3E Antibody - Western blot analysis of extracts of various cell lines, using CD3E Antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 1s.
CD3E Antibody - Immunohistochemistry of paraffin-embedded mouse thymus using CD3E antibody at dilution of 1:100 (40x lens).
CD3E Antibody - Immunohistochemistry of paraffin-embedded mouse spleen using CD3E antibody at dilution of 1:100 (40x lens).
CD3E Antibody - Western blot analysis of extracts of various cell lines, using CD3E Antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 1s.
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Polyclonal Rabbit anti‑Human CD3E Antibody (IHC, WB) LS‑C747519

Polyclonal Rabbit anti‑Human CD3E Antibody (IHC, WB) LS‑C747519

Antibody:
CD3E Rabbit anti-Human Polyclonal Antibody
Application:
IHC, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C747519-20
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
CD3E Rabbit anti-Human Polyclonal Antibody
Application:
IHC, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
CD3E antibody LS-C747519 is an unconjugated rabbit polyclonal antibody to CD3E from human. It is reactive with human, mouse and rat. Validated for IHC and WB.
Target
Human CD3E
Synonyms
CD3E | CD3e antigen | CD3-epsilon | T-cell receptor epsilon chain | TCRE | T-cell receptors epsilon chain | T3E | CD3 Epsilon | CD3
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 100 to the C-Terminus of human CD3E (NP_000724.1). PRGSKPEDANFYLYLRARVCENCMEMDVMSVATIVIVDICITGGLLLLVYYWSKNRKAKAKPVTRGAGAGGRQRGQNKERPPPVPNPDYEPIRKGQRDLYSGLNQRRI
Applications
  • IHC (1:50 - 1:200)
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
The predicted MW is 23kDa, while the observed MW by Western blot was 23kDa, 25kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CD3E

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/26/2024