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Catalog Number Size Price
LS-C796727-100 100 µg $539 
CD3E Antibody - IHC staining of FFPE human tonsil using anti-CD3E clone UMAB48 mouse monoclonal antibody at 1:200 and detection with Polink2 Broad HRP DAB.requires heat-induced epitope retrieval with Citrate pH6.O. The image shows strong membranous and cytoplasmic staining.
CD3E Antibody - Immunohistochemical staining of paraffin-embedded mouse ascending colon tissue using anti-CD3 clone UMAB54 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer. (pH8.5) at 120°C for 3 min. (1:300).
CD3E Antibody - Immunohistochemical staining of paraffin-embedded mouse spleen tissue using anti-CD3E clone UMAB54 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer. (pH8.5) at 120 oC for 3 min. (1:300).
CD3E Antibody - Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-UM500048 mouse monoclonal antibody.  heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
CD3E Antibody - Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-UM500048 mouse monoclonal antibody.  heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
CD3E Antibody - Immunohistochemical staining of paraffin-embedded Human tonsil using anti-UM500048mouse monoclonal antibody.  heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
CD3E Antibody - Immunofluorescent staining of Jurkat cells using CD3E mouse monoclonal antibody  green). Actin filaments were labeled with TRITC-phalloidin. (red), and nuclear with DAPI. (blue). The three-color overlay image is located at the bottom-right corner.
CD3E Antibody - Western blot analysis of extracts. (10ug) from 10 Human tissue by using anti-CD3E monoclonal antibody at 1:500. (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
CD3E Antibody - Western blot of cell lysates. (35ug) from 9 different cell lines. (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549, 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7). Diluation: 1:500
CD3E Antibody - IHC staining of FFPE human tonsil using anti-CD3E clone UMAB48 mouse monoclonal antibody at 1:200 and detection with Polink2 Broad HRP DAB.requires heat-induced epitope retrieval with Citrate pH6.O. The image shows strong membranous and cytoplasmic staining.
CD3E Antibody - Immunohistochemical staining of paraffin-embedded mouse ascending colon tissue using anti-CD3 clone UMAB54 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer. (pH8.5) at 120°C for 3 min. (1:300).
CD3E Antibody - Immunohistochemical staining of paraffin-embedded mouse spleen tissue using anti-CD3E clone UMAB54 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer. (pH8.5) at 120 oC for 3 min. (1:300).
CD3E Antibody - Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-UM500048 mouse monoclonal antibody.  heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
CD3E Antibody - Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-UM500048 mouse monoclonal antibody.  heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
CD3E Antibody - Immunohistochemical staining of paraffin-embedded Human tonsil using anti-UM500048mouse monoclonal antibody.  heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
CD3E Antibody - Immunofluorescent staining of Jurkat cells using CD3E mouse monoclonal antibody  green). Actin filaments were labeled with TRITC-phalloidin. (red), and nuclear with DAPI. (blue). The three-color overlay image is located at the bottom-right corner.
CD3E Antibody - Western blot analysis of extracts. (10ug) from 10 Human tissue by using anti-CD3E monoclonal antibody at 1:500. (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
CD3E Antibody - Western blot of cell lysates. (35ug) from 9 different cell lines. (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549, 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7). Diluation: 1:500
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Monoclonal Mouse anti‑Human CD3E Antibody (clone UMAB54, Carrier‑free, IHC, IF, WB) LS‑C796727

Monoclonal Mouse anti‑Human CD3E Antibody (clone UMAB54, Carrier‑free, IHC, IF, WB) LS‑C796727

Antibody:
CD3E Mouse anti-Human Monoclonal (Carrier-free) (UMAB54) Antibody
Application:
IHC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Carrier-free
Other formats:
Price
Catalog Number
$539
LS-C796727-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
CD3E Mouse anti-Human Monoclonal (Carrier-free) (UMAB54) Antibody
Application:
IHC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Carrier-free
Other formats:

Specifications

Description
CD3E antibody LS-C796727 is an unconjugated mouse monoclonal antibody to human CD3E. Validated for IF, IHC and WB.
Target
Human CD3E
Synonyms
CD3E | CD3e antigen | CD3-epsilon | T-cell receptor epsilon chain | TCRE | T-cell receptors epsilon chain | T3E | CD3 Epsilon | CD3
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2b Monoclonal
Clone
UMAB54
Conjugations
Unconjugated
Purification
Affinity chromatography
Modifications
Carrier-free. Also available Unmodified.
Immunogen
Full length human recombinant protein of human CD3E(NP_000724) produced in HEK293T cell.
Specificity
The specificity of this antibody has been validated by testing against a high-density Protein Microarray containing more than 17,000 recombinant proteins.
Applications
  • IHC (1:100)
  • Immunofluorescence (1:100)
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
PBS, pH 7.3, 8% Trehalose
Reconstitution
Reconstitute with 100ul distilled water to a final concentration of 1mg/ml. To use this carrier-free antibody for conjugation experiment, we strongly recommend you to perform another round of desalting process.
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CD3E

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Requested From: United States
Date Requested: 11/22/2024