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Catalog Number Size Price
LS-C343957-10 10 µg $318 
LS-C343957-100 100 µg (0.5 mg/ml) $470 
CD3E Antibody - CD3 epsilon antibody IHC-frozen: Rat Spleen Tissue.
CD3E Antibody - CD3 epsilon antibody IHC-frozen: Mouse Spleen Tissue.
CD3E Antibody - IHC analysis of CD3 Epsilon using anti-CD3 Epsilon antibody. CD3 Epsilon was detected in frozen section of rat spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CD3 Epsilon Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CD3E Antibody - CD3 epsilon antibody IHC-paraffin: Human Tonsil Tissue.
CD3E Antibody - CD3 epsilon antibody IHC-paraffin: Mouse Spleen Tissue.
CD3E Antibody - CD3 epsilon antibody IHC-paraffin: Rat Spleen Tissue.
CD3E Antibody - IF analysis of CD3E/CD68 using anti-CD3E/CD68 antibody CD3E/CD68 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/mL rabbit anti-CD3E Antibody/ mouse anti CD68 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG and Cy3 Conjugated Avidin were used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
CD3E Antibody - CD3 epsilon antibody Western blot. All lanes: Anti CD3 Epsilon at 0.5 ug/ml. Lane 1: JURKAT Whole Cell Lysate at 40 ug. Lane 2: CEM Whole Cell Lysate at 40 ug. Predicted band size: 23 kD. Observed band size: 23 kD.
CD3E Antibody - CD3 epsilon antibody Western blot. All lanes: Anti CD3 Epsilon at 0.5 ug/ml. WB : Recombinant Human CD3epsilon Protein 0.5ng . Predicted band size: 40 kD. Observed band size: 40 kD.
CD3E Antibody - CD3 epsilon antibody IHC-frozen: Rat Spleen Tissue.
CD3E Antibody - CD3 epsilon antibody IHC-frozen: Mouse Spleen Tissue.
CD3E Antibody - IHC analysis of CD3 Epsilon using anti-CD3 Epsilon antibody. CD3 Epsilon was detected in frozen section of rat spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CD3 Epsilon Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CD3E Antibody - CD3 epsilon antibody IHC-paraffin: Human Tonsil Tissue.
CD3E Antibody - CD3 epsilon antibody IHC-paraffin: Mouse Spleen Tissue.
CD3E Antibody - CD3 epsilon antibody IHC-paraffin: Rat Spleen Tissue.
CD3E Antibody - IF analysis of CD3E/CD68 using anti-CD3E/CD68 antibody CD3E/CD68 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/mL rabbit anti-CD3E Antibody/ mouse anti CD68 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG and Cy3 Conjugated Avidin were used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
CD3E Antibody - CD3 epsilon antibody Western blot. All lanes: Anti CD3 Epsilon at 0.5 ug/ml. Lane 1: JURKAT Whole Cell Lysate at 40 ug. Lane 2: CEM Whole Cell Lysate at 40 ug. Predicted band size: 23 kD. Observed band size: 23 kD.
CD3E Antibody - CD3 epsilon antibody Western blot. All lanes: Anti CD3 Epsilon at 0.5 ug/ml. WB : Recombinant Human CD3epsilon Protein 0.5ng . Predicted band size: 40 kD. Observed band size: 40 kD.
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Polyclonal Rabbit anti‑Human CD3E Antibody (aa23‑207, IHC, WB) LS‑C343957

Polyclonal Rabbit anti‑Human CD3E Antibody (aa23‑207, IHC, WB) LS‑C343957

Note: This antibody replaces LS-C357768
Antibody:
CD3E Rabbit anti-Human Polyclonal (aa23-207) Antibody
Application:
IHC, IHC-P, IHC-Fr, ICC, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C343957-10
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
CD3E Rabbit anti-Human Polyclonal (aa23-207) Antibody
Application:
IHC, IHC-P, IHC-Fr, ICC, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
CD3E antibody LS-C343957 is an unconjugated rabbit polyclonal antibody to CD3E (aa23-207) from human. It is reactive with human, mouse and rat. Validated for ICC, IHC and WB. Cited in 1 publication.
Target
Human CD3E
Synonyms
CD3E | CD3e antigen | CD3-epsilon | T-cell receptor epsilon chain | TCRE | T-cell receptors epsilon chain | T3E | CD3 Epsilon | CD3
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E.coli-derived human CD3 epsilon recombinant protein (Position: D23-I207). Human CD3 epsilon shares 65% amino acid (aa) sequence identity with mouse CD3 epsilon.
Epitope
aa23-207
Applications
  • IHC
  • IHC - Paraffin
  • IHC - Frozen
  • ICC
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CD3E

LSBio Ratings

CD3E Antibody (aa23-207) for IHC, ICC, WB/Western LS-C343957 has an LSBio Rating of
Publications (4)

Publications (1)

Inhibition of STAT3 augments antitumor efficacy of anti-CTLA-4 treatment against prostate cancer. Kristina Witt , Susan Evans-Axelsson, Andreas Lundqvist, Martin Johansson, Anders Bjartell, Rebecka Hellsten. Cancer immunology, immunotherapy : CII. 2021 Nov;;70:3155-3166.

Customer Reviews (0)


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Requested From: United States
Date Requested: 11/22/2024