Reactivity
Mouse
(tested or 100% immunogen sequence identity)
Clonality
IgG2a
Monoclonal
Conjugations
Allophycocyanin.
Also available
Unconjugated or conjugated with
RPE,
FITC.
Purification
Affinity purified
Specificity
Recognizes the mouse CD3 antigen which is expressed by mature T cells. For optimal staining incubations should be performed at room temperature.
Applications
- Flow Cytometry (1:1)
- Applications tested for the base form of this product only
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells or 100 ul whole blood. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.
Presentation
PBS, 0.09% Sodium Azide, 5% Sucrose, 1% BSA
Reconstitution
Reconstitute with distilled water.
Storage
Store at 4°C. Do not freeze. Store undiluted. Protect from light. Microcentrifugation recommended if solution contains precipitate.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee