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Catalog Number Size Price
LS-C331610-20 20 µl $275 
LS-C331610-100 100 µl $389 
CD274 / B7-H1 / PD-L1 Antibody - Western blot analysis of extracts of various cell lines, using CD274 antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
CD274 / B7-H1 / PD-L1 Antibody - Western blot analysis of extracts of various cell lines.
CD274 / B7-H1 / PD-L1 Antibody - Western blot analysis of extracts of various cell lines, using CD274 antibody.
CD274 / B7-H1 / PD-L1 Antibody - Western blot analysis of extracts of various cell lines, using CD274 antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
CD274 / B7-H1 / PD-L1 Antibody - Western blot analysis of extracts of various cell lines.
CD274 / B7-H1 / PD-L1 Antibody - Western blot analysis of extracts of various cell lines, using CD274 antibody.
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Polyclonal Rabbit anti‑Human CD274 / B7‑H1 / PD‑L1 Antibody (IHC, WB) LS‑C331610

Polyclonal Rabbit anti‑Human CD274 / B7‑H1 / PD‑L1 Antibody (IHC, WB) LS‑C331610

Antibody:
CD274 / B7-H1 / PD-L1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C331610-20
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
CD274 / B7-H1 / PD-L1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
PD-L1 antibody LS-C331610 is an unconjugated rabbit polyclonal antibody to PD-L1 (CD274 / B7-H1) from human. It is reactive with human, mouse and rat. Validated for IHC and WB.
Target
Human CD274 / B7-H1 / PD-L1
Synonyms
CD274 | B7-H | B7 homolog 1 | B7-H1 | B7H1 | CD274 molecule | CD274 antigen | PDCD1 ligand 1 | PDL1 | PD-L1 | PDCD1L1 | PDCD1LG1 | Programmed death ligand 1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 19-238 of human CD274 (NP_054862.1). FTVTVPKDLYVVEYGSNMTIECKFPVEKQLDLAALIVYWEMEDKNIIQFVHGEEDLKVQHSSYRQRARLLKDQLSLGNAALQITDVKLQDAGVYRCMISYGGADYKRITVKVNAPYNKINQRILVVDPVTSEHELTCQAEGYPKAEVIWTSSDHQVLSGKTTTTNSKREEKLFNVTSTLRINTTTNEIFYCTFRRLDPEENHTAELVIPELPLAHPPNER
Specificity
Human B7-H1 / PD-L1 / CD274
Applications
  • IHC
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
The predicted MW is 20kDa/33kDa, while the observed MW by Western blot was 43kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CD274 / B7-H1 / PD-L1
Q9NZQ7 NM_014143 NP_054862.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/12/2024