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Catalog Number	Size	Price
LS-C331610-20	20 µl	$275
LS-C331610-100	100 µl	$389

CD274 / B7-H1 / PD-L1 Antibody - Western blot analysis of extracts of various cell lines, using CD274 antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.

CD274 / B7-H1 / PD-L1 Antibody - Western blot analysis of extracts of various cell lines.

CD274 / B7-H1 / PD-L1 Antibody - Western blot analysis of extracts of various cell lines, using CD274 antibody.

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Polyclonal Rabbit anti‑Human CD274 / B7‑H1 / PD‑L1 Antibody (IHC, WB) LS‑C331610

Name: Anti-CD274 / B7-H1 / PD-L1 Antibody | Rabbit anti-Human Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C331610-20
Price: 275.0000 USD
Availability: InStock

Submit Review LSBio Guarantee

Antibody:

CD274 / B7-H1 / PD-L1 Rabbit anti-Human Polyclonal Antibody

Application:

IHC, WB

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

$275

LS-C331610-20

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

CD274 / B7-H1 / PD-L1 Rabbit anti-Human Polyclonal Antibody

Application:

IHC, WB

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

Specifications

Description

PD-L1 antibody LS-C331610 is an unconjugated rabbit polyclonal antibody to PD-L1 (CD274 / B7-H1) from human. It is reactive with human, mouse and rat. Validated for IHC and WB.

Target

Human CD274 / B7-H1 / PD-L1

Synonyms

CD274 | B7-H | B7 homolog 1 | B7-H1 | B7H1 | CD274 molecule | CD274 antigen | PDCD1 ligand 1 | PDL1 | PD-L1 | PDCD1L1 | PDCD1LG1 | Programmed death ligand 1

See All CD274 / B7-H1 / PD-L1 Antibodies

Host

Rabbit

Reactivity

Human, Mouse, Rat (tested or 100% immunogen sequence identity)

Clonality

IgG Polyclonal

Conjugations

Unconjugated

Purification

Affinity purified

Modifications

Unmodified

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 19-238 of human CD274 (NP_054862.1). FTVTVPKDLYVVEYGSNMTIECKFPVEKQLDLAALIVYWEMEDKNIIQFVHGEEDLKVQHSSYRQRARLLKDQLSLGNAALQITDVKLQDAGVYRCMISYGGADYKRITVKVNAPYNKINQRILVVDPVTSEHELTCQAEGYPKAEVIWTSSDHQVLSGKTTTTNSKREEKLFNVTSTLRINTTTNEIFYCTFRRLDPEENHTAELVIPELPLAHPPNER

Specificity

Human B7-H1 / PD-L1 / CD274

Applications

IHC
Western blot (1:500 - 1:2000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

The predicted MW is 20kDa/33kDa, while the observed MW by Western blot was 43kDa.

Presentation

PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol

Storage

Store at -20°C. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About CD274 / B7-H1 / PD-L1