Target
Human CD274 / B7-H1 / PD-L1
Synonyms
CD274 | B7-H | B7 homolog 1 | B7-H1 | B7H1 | CD274 molecule | CD274 antigen | PDCD1 ligand 1 | PDL1 | PD-L1 | PDCD1L1 | PDCD1LG1 | Programmed death ligand 1
Reactivity
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Peptide Column Affinity Chromatography
Immunogen
A 17 AA synthetic peptide near the center of human PD-L1. The immunogen is located within aa 60-110 of PD-L1.
Specificity
PD-L1 antibody has no cross-reactivity to PD-L2.
Applications
- Immunofluorescence
- Western blot
- Flow Cytometry
- ELISA
Usage
PD-L1 antibody can be used for detection of PD-L1 by Western blot at 0.5 - 1 ug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 ug/mL. For immunofluorescence start at 20 ug/mL. Flow cytometry at 0.5 ug/ml. Antibody validated: Western Blot in human samples; Immunohistochemistry in human and rat samples; Immunofluorescence in human and rat samples and Flow Cytometry in mouse samples. All other applications and species not yet tested. Western Blot: Predicted: 32 kDa Observed: 37 kDa
Presentation
PBS containing 0.02% Sodium Azide
Storage
Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CD274 / B7-H1 / PD-L1