Human CD274 / B7-H1 / PD-L1

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

ANC6H1

R. Phycoerythrin. Also available Unconjugated or conjugated with Biotin, FITC.

Protein A purified

Unmodified

Recombinant human CD274(B7-H1).

Recombinant CD274 and native cell surface CD274 expressed on stimulated PBMC.

• Flow Cytometry
• ELISA
• Applications tested for the base form of this product only

Ficoll prepared human peripheral blood mononuclear cells (PBMC) were stimulated overnight in culture with 5 ug/ml PHA. Five x 105 cells per tube were washed and pre incubated 5 minutes with 20^l of 250^g/ml human IgG (to block non specific binding), after which they were incubated 45 minutes on ice with 80 ^l of anti-human CD274 at a dilution factor of 1:50 (20 ^g/ml). They were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.67 log10 fluorescent units when compared to a Mouse IgG1/R-PE negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 ^l of 0.5 mg/ml anti-CD274 antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 500 mM KCl, 0.04% Sodium Azide, 15% Glycerol, 0.2% BSA

Store at 4°C. Do not freeze. Protect from light.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q9NZQ7 NCBI: NM_014143 NP_054862.1