Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Forgot password?
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Quick Order
Products
Antibodies
ELISA and Assay Kits
Research Areas
Infectious Disease
Resources
Purchasing
Reference Material
Contact Us
Location
Corporate Headquarters
Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
United States
Telephone Numbers
Customer Service: (800) 227-6666 / (650) 697-3600
Contact Us
Additional Contact Details
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Forgot password?
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Quick Order
| Catalog Number | Size | Price |
|---|---|---|
| LS-C669076-0.1 | 0.1 mg (1 mg/ml) | $502 |
![CD274 / B7-H1 / PD-L1 Antibody - Immunofluorescence of PD-L1 in transfected HEK293 cells with PD-L1 antibody at 2 ug/mL. Red: PDL1 Antibody [4F2] Blue: DAPI staining](https://lsbio-7d62.kxcdn.com/image2/cd274-b7-h1-pd-l1-antibody-clone-4f2-ls-c669076/484801_3815940.gif)
![CD274 / B7-H1 / PD-L1 Antibody - Immunofluorescence of PD-L1 in human tonsil tissue with PD-L1 antibody at 2 ug/mL. Red: PDL1 Antibody [4F2] Blue: DAPI staining](https://lsbio-7d62.kxcdn.com/image2/cd274-b7-h1-pd-l1-antibody-clone-4f2-ls-c669076/484800_3815929.gif)
![CD274 / B7-H1 / PD-L1 Antibody - Immunofluorescence of PD-L1 in human stomach carcinoma tissue with PD-L1 antibody at 2 ug/mL. Red: PDL1 Antibody [4F2] Blue: DAPI staining](https://lsbio-7d62.kxcdn.com/image2/cd274-b7-h1-pd-l1-antibody-clone-4f2-ls-c669076/484799_3815917.gif)
1 of 8
2 of 8
3 of 8
4 of 8
5 of 8
6 of 8
7 of 8
8 of 8
Monoclonal Mouse anti‑Human CD274 / B7‑H1 / PD‑L1 Antibody (clone 4F2, IHC, IF, WB) LS‑C669076
Monoclonal Mouse anti‑Human CD274 / B7‑H1 / PD‑L1 Antibody (clone 4F2, IHC, IF, WB) LS‑C669076
Antibody:
CD274 / B7-H1 / PD-L1 Mouse anti-Human Monoclonal (4F2) Antibody
Application:
IHC-P, ICC, IF, WB, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
CD274 / B7-H1 / PD-L1 Mouse anti-Human Monoclonal (4F2) Antibody
Application:
IHC-P, ICC, IF, WB, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
PD-L1 antibody LS-C669076 is an unconjugated mouse monoclonal antibody to human PD-L1 (CD274 / B7-H1). Validated for ELISA, Flow, ICC, IF, IHC and WB.
Target
Human CD274 / B7-H1 / PD-L1
Synonyms
CD274 | B7-H | B7 homolog 1 | B7-H1 | B7H1 | CD274 molecule | CD274 antigen | PDCD1 ligand 1 | PDL1 | PD-L1 | PDCD1L1 | PDCD1LG1 | Programmed death ligand 1
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Clone
4F2
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Immunogen
The extracellular domain of human PD-L1.
Applications
- IHC - Paraffin
- ICC
- Immunofluorescence
- Western blot
- Flow Cytometry
- ELISA
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
PD-L1 antibody can be used for detection of PD-L1 by Western blot at 0.25 - 0.5 ug/mL. Antibody can also be used for immunohistochemistry starting at 2 - 5 ug/mL. For immunofluorescence start at 5 ug/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in mouse samples. All other applications and species not yet tested. Western Blot: Predicted: 32 kDa Observed: 45 kDa
Presentation
PBS containing 0.02% Sodium Azide
Storage
Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About CD274 / B7-H1 / PD-L1
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Flow Cytometry
Species:
Human
Applications:
Flow Cytometry, ELISA
Species:
Human
Applications:
Flow Cytometry, ELISA
Species:
Human
Applications:
Flow Cytometry, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Mouse
Applications:
IHC, IHC - Frozen, Flow Cytometry
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/2/2024
Date Requested: 12/2/2024
