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Catalog Number Size Price
LS-C669081-0.1 0.1 mg (1 mg/ml) $502 
CD274 / B7-H1 / PD-L1 Antibody - Immunohistochemistry of PD-L1 in human stomach carcinoma tissue with PD-L1 antibody at 5 ug/mL.
CD274 / B7-H1 / PD-L1 Antibody - Immunohistochemistry of PD-L1 in human tonsil tissue with PD-L1 antibody at 5 ug/mL.
CD274 / B7-H1 / PD-L1 Antibody - Immunocytochemistry of PD-L1 in transfected HEK293 cells with PD-L1 antibody at 1 ug/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 1 ug/mL.
CD274 / B7-H1 / PD-L1 Antibody - Immunofluorescence of PD-L1 in transfected HEK293 cells with PD-L1 antibody at 2 ug/mL. Red: PDL1 Antibody [1F11] Blue: DAPI staining
CD274 / B7-H1 / PD-L1 Antibody - Immunofluorescence of PD-L1 in human tonsil tissue with PD-L1 antibody at 2 ug/mL. Red: PDL1 Antibody [1F11] Blue: DAPI staining
CD274 / B7-H1 / PD-L1 Antibody - Immunofluorescence of PD-L1 in human stomach carcinoma tissue with PD-L1 antibody at 2 ug/mL. Red: PDL1 Antibody [1F11] Blue: DAPI staining
CD274 / B7-H1 / PD-L1 Antibody - Western blot analysis of PD-L1 in overexpressing HEK293 cells PD-L1 antibody at 0.25 and 0.5 ug/ml
CD274 / B7-H1 / PD-L1 Antibody - Immunohistochemistry of PD-L1 in human stomach carcinoma tissue with PD-L1 antibody at 5 ug/mL.
CD274 / B7-H1 / PD-L1 Antibody - Immunohistochemistry of PD-L1 in human tonsil tissue with PD-L1 antibody at 5 ug/mL.
CD274 / B7-H1 / PD-L1 Antibody - Immunocytochemistry of PD-L1 in transfected HEK293 cells with PD-L1 antibody at 1 ug/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 1 ug/mL.
CD274 / B7-H1 / PD-L1 Antibody - Immunofluorescence of PD-L1 in transfected HEK293 cells with PD-L1 antibody at 2 ug/mL. Red: PDL1 Antibody [1F11] Blue: DAPI staining
CD274 / B7-H1 / PD-L1 Antibody - Immunofluorescence of PD-L1 in human tonsil tissue with PD-L1 antibody at 2 ug/mL. Red: PDL1 Antibody [1F11] Blue: DAPI staining
CD274 / B7-H1 / PD-L1 Antibody - Immunofluorescence of PD-L1 in human stomach carcinoma tissue with PD-L1 antibody at 2 ug/mL. Red: PDL1 Antibody [1F11] Blue: DAPI staining
CD274 / B7-H1 / PD-L1 Antibody - Western blot analysis of PD-L1 in overexpressing HEK293 cells PD-L1 antibody at 0.25 and 0.5 ug/ml
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Monoclonal Mouse anti‑Human CD274 / B7‑H1 / PD‑L1 Antibody (clone 1F11, IHC, IF, WB) LS‑C669081

Monoclonal Mouse anti‑Human CD274 / B7‑H1 / PD‑L1 Antibody (clone 1F11, IHC, IF, WB) LS‑C669081

Antibody:
CD274 / B7-H1 / PD-L1 Mouse anti-Human Monoclonal (1F11) Antibody
Application:
IHC-P, ICC, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$502
LS-C669081-0.1
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
CD274 / B7-H1 / PD-L1 Mouse anti-Human Monoclonal (1F11) Antibody
Application:
IHC-P, ICC, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
PD-L1 antibody LS-C669081 is an unconjugated mouse monoclonal antibody to human PD-L1 (CD274 / B7-H1). Validated for ELISA, ICC, IF, IHC and WB.
Target
Human CD274 / B7-H1 / PD-L1
Synonyms
CD274 | B7-H | B7 homolog 1 | B7-H1 | B7H1 | CD274 molecule | CD274 antigen | PDCD1 ligand 1 | PDL1 | PD-L1 | PDCD1L1 | PDCD1LG1 | Programmed death ligand 1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
1F11
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Immunogen
The extracellular domain of human PD-L1.
Applications
  • IHC - Paraffin
  • ICC
  • Immunofluorescence
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
PD-L1 antibody can be used for detection of PD-L1 by Western blot at 1 - 2 ug/mL. Antibody can also be used for immunohistochemistry starting at 2 - ug/mL. For immunofluorescence start at 5 ug/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: Predicted: 32 kDa Observed: 45 kDa
Presentation
PBS containing 0.02% Sodium Azide
Storage
Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CD274 / B7-H1 / PD-L1
Q9NZQ7 NM_014143 NP_054862.1

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 11/12/2024