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| Catalog Number | Size | Price |
|---|---|---|
| LS-C389866-0.1 | 0.1 ml | $371 |
| LS-C389866-0.5 | 0.5 ml | $574 |
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Monoclonal Mouse anti‑Human CD20 Antibody (clone L26 + IGEL/733, IHC, IF) LS‑C389866
Monoclonal Mouse anti‑Human CD20 Antibody (clone L26 + IGEL/733, IHC, IF) LS‑C389866
Antibody:
CD20 Mouse anti-Human Monoclonal (L26 + IGEL/733) Antibody
Application:
IHC, IHC-P, IF, Flo
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
CD20 Mouse anti-Human Monoclonal (L26 + IGEL/733) Antibody
Application:
IHC, IHC-P, IF, Flo
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified
Specifications
Description
CD20 antibody LS-C389866 is an unconjugated mouse monoclonal antibody to CD20 from human. It is reactive with human and monkey. Validated for Flow, IF and IHC.
Target
Human CD20
Synonyms
MS4A1 | B-lymphocyte antigen CD20 | B1 | Bp35 | CD20 | CD20 antigen | CD20 receptor | LEU-16 | CVID5
Host
Mouse
Reactivity
Human, Monkey
(tested or 100% immunogen sequence identity)
Non-Reactivity
Rat, Bovine, Dog, Pig
Clonality
IgG2a,k
Monoclonal
Clone
L26 + IGEL/733
Conjugations
Unconjugated
Purification
Bioreactor concentrate
Modifications
Unmodified
Immunogen
Human tonsil B cells (L26) and recombinant protein (IGEL/733) were used as the immunogen for this CD20 antibody cocktail.
Specificity
Human CD20
Applications
- IHC
- IHC - Paraffin (1:100 - 1:200)
- Immunofluorescence (1:50 - 1:100)
- Flow Cytometry
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
This antibody recognizes a protein of 33-37kDa, identified as CD20 (Workshop V. Code CD20.12). The antibody recognizes the extracellular domain of the protein. The epitope is similar to or identical to that recognized by other CD20 antibodies including Leu-16 and B1. This antibody can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood, Bcell localization in tissues and B lymphocyte purification by immunosorbent methods. CD20 is a non-Ig differentiation antigen of Bcells and its expression is restricted to normal and neoplastic Bcells, being absent from all other leukocytes and tissues. It is expressed by pre Bcells and persists during all stages of Bcell maturation but is lost upon terminal differentiation into plasma cells. Protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. CD20 is not glycosylated in resting Bcells and its cytoplasmic domains are differentially phosphorylated upon activation. It acts as a calcium channel involved in Bcell activation and cell cycle progression. Variations in protocols, secondaries and substrates may require the CD20 antibody cocktail to be titered up or down for optimal performance. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Presentation
Bioreactor concentrate, 0.05% sodium azide
Storage
Store at 2°C to 8°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
About CD20
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Featured Products
Species:
Mouse, Human
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Frozen, Immunoprecipitation, Flow Cytometry, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/2/2024
Date Requested: 12/2/2024
