Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human Eotaxin-3 (2 ug/ml), a concentration of 1-5 ug/ml of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 ul/well), a concentration of 1-2 ug/ml of this antibody will detect at least 1000 pg/ml of recombinant human Eotaxin-3 when used with biotinylated antigen affinity purified anti-human Eotaxin-3 (
LS-C104390) as the detection antibody at a concentration of approximately 0.25-0.50 ug/ml. Western Blot: To detect hEotaxin-3 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEotaxin-3 is 0.25-0.5 ng/lane, under reducing or non-reducing conditions.