Target
Human CCL21 / SLC
Synonyms
CCL21 | 6Ckine | Beta chemokine exodus-2 | C-C motif chemokine 21 | Beta-chemokine exodus-2 | CKb9 | ECL | Small-inducible cytokine A21 | SCYA21 | SLC | Exodus-2 | TCA4
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Biotin.
Also available
Unconjugated.
Purification
Immunoaffinity purified
Immunogen
E.coli derived recombinant Human Exodus-2.
Specificity
Human Exodus-2
Applications
- Western blot (0.1 - 0.2 µg/ml)
- ELISA (0.25 - 1 µg/ml)
- Applications tested for the base form of this product only
Usage
ELISA: To detect hExodus-2 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hExodus-2. To detect hExodus-2 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human Exodus-2 (LS-B7413) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hExodus-2. Western Blot: To detect hExodus-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hExodus-2 is 1.5-3 ng/lane, under either reducing or non-reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from PBS, pH 7.2
Reconstitution
Sterile PBS containing 0.1% BSA
Storage
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee