Target
Bovine CCL2 / MCP1
Synonyms
CCL2 | C-C motif chemokine 2 | Chemokine (C-C motif) ligand 2 | GDCF-2 | HC11 | HSMCR30 | MCP1 | SCYA2 | Small-inducible cytokine A2 | Monocyte secretory protein JE | MCAF | MCP-1 | Monocyte chemotactic protein 1 | SMC-CF
Reactivity
Bovine
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Lyophilized
Immunogen
This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to the 76 amino acids of the mature bovine CCL2 protein.
Specificity
This antibody is specific for bovine CCL2 protein. A BLAST analysis was used to suggest cross-reactivity with CCL2 from bovine sources based on 100% homology with the immunizing sequence. Partial reactivity is expected against Sus scrofa 85.5% and Bos Taurus 81.1%. Cross-reactivity with CCL2 from other sources has not been determined.
Applications
- Western blot (1 µg/ml)
- ELISA (1:4500)
Usage
This protein-A purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 8.8 kD in size corresponding to bovine CCL2 protein by western blotting in the appropriate cell lysate or extract.
Presentation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze/thaw cycles. Store undiluted.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee