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Catalog Number Size Price
LS-C154026-100 100 µg (1 mg/ml) $567 
CCL2 / MCP1 Antibody - Anti-Bovine CCL2 Antibody - Western Blot. Western blot of protein-A purified anti-bovine CCL2 antibody shows detection of recombinant bovine CCL2 at 8.8kD (arrow) raised in yeast. Protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1:10), and probed with Inc. Anti-bovine CCL2. After washing, membrane was probed with Dylight 649 Conjugated Anti-Rabbit IgG (H&L) (Donkey) Antibody (.

Polyclonal Rabbit anti‑Bovine CCL2 / MCP1 Antibody (Lyophilized, WB) LS‑C154026

Polyclonal Rabbit anti‑Bovine CCL2 / MCP1 Antibody (Lyophilized, WB) LS‑C154026

Antibody:
CCL2 / MCP1 Rabbit anti-Bovine Polyclonal (Lyophilized) Antibody
Application:
WB, ELISA
Reactivity:
Bovine
Format:
Unconjugated, Lyophilized
Price
Catalog Number
$567
LS-C154026-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
CCL2 / MCP1 Rabbit anti-Bovine Polyclonal (Lyophilized) Antibody
Application:
WB, ELISA
Reactivity:
Bovine
Format:
Unconjugated, Lyophilized

Specifications

Description
MCP1 antibody LS-C154026 is an unconjugated rabbit polyclonal antibody to bovine MCP1 (CCL2). Validated for ELISA and WB.
Target
Bovine CCL2 / MCP1
Synonyms
CCL2 | C-C motif chemokine 2 | Chemokine (C-C motif) ligand 2 | GDCF-2 | HC11 | HSMCR30 | MCP1 | SCYA2 | Small-inducible cytokine A2 | Monocyte secretory protein JE | MCAF | MCP-1 | Monocyte chemotactic protein 1 | SMC-CF
Host
Rabbit
Reactivity
Bovine (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Lyophilized
Immunogen
This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to the 76 amino acids of the mature bovine CCL2 protein.
Specificity
This antibody is specific for bovine CCL2 protein. A BLAST analysis was used to suggest cross-reactivity with CCL2 from bovine sources based on 100% homology with the immunizing sequence. Partial reactivity is expected against Sus scrofa 85.5% and Bos Taurus 81.1%. Cross-reactivity with CCL2 from other sources has not been determined.
Applications
  • Western blot (1 µg/ml)
  • ELISA (1:4500)
Usage
This protein-A purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 8.8 kD in size corresponding to bovine CCL2 protein by western blotting in the appropriate cell lysate or extract.
Presentation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze/thaw cycles. Store undiluted.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CCL2 / MCP1
P13500 NM_002982 NP_002973.1

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/24/2024