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Catalog Number Size Price
LS-B10540-200 200 µl $460 
CCL2 / MCP1 Antibody - Anti-CCL2 / MCP1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:75.
CCL2 / MCP1 Antibody - Confocal immunofluorescence of CCL2 Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
CCL2 / MCP1 Antibody - Immunofluorescence of CCL2 Antibody with HeLa cells. 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.
CCL2 / MCP1 Antibody - Western blot of CCL2 Antibody in HeLa cell line lysates (35 ug/lane). CCL2 (arrow) was detected using the purified antibody.
CCL2 / MCP1 Antibody - Flow cytometric of HeLa cells using CCL2 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
CCL2 / MCP1 Antibody - Anti-CCL2 / MCP1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:75.
CCL2 / MCP1 Antibody - Confocal immunofluorescence of CCL2 Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
CCL2 / MCP1 Antibody - Immunofluorescence of CCL2 Antibody with HeLa cells. 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.
CCL2 / MCP1 Antibody - Western blot of CCL2 Antibody in HeLa cell line lysates (35 ug/lane). CCL2 (arrow) was detected using the purified antibody.
CCL2 / MCP1 Antibody - Flow cytometric of HeLa cells using CCL2 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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IHC‑plus™ Polyclonal Rabbit anti‑Human CCL2 / MCP1 Antibody (aa62‑89, IHC, IF, WB) LS‑B10540

IHC‑plus™ Polyclonal Rabbit anti‑Human CCL2 / MCP1 Antibody (aa62‑89, IHC, IF, WB) LS‑B10540

Note: This antibody replaces LS-C168450
Antibody:
CCL2 / MCP1 Rabbit anti-Human Polyclonal (aa62-89) Antibody
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B10540-200
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
CCL2 / MCP1 Rabbit anti-Human Polyclonal (aa62-89) Antibody
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
MCP1 antibody LS-B10540 is an unconjugated rabbit polyclonal antibody to human MCP1 (CCL2) (aa62-89). Validated for Flow, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human CCL2 / MCP1
Synonyms
CCL2 | C-C motif chemokine 2 | Chemokine (C-C motif) ligand 2 | GDCF-2 | HC11 | HSMCR30 | MCP1 | SCYA2 | Small-inducible cytokine A2 | Monocyte secretory protein JE | MCAF | MCP-1 | Monocyte chemotactic protein 1 | SMC-CF
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Monkey (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Epitope
aa62-89
Specificity
This CCL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-89 amino acids from the C-terminal region of human CCL2.
Applications
  • IHC
  • IHC - Paraffin (1:75)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CCL2 / MCP1
P13500 NM_002982 NP_002973.1

Validation

CCL2 / MCP1 Antibody - Anti-CCL2 / MCP1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:75.
Anti-CCL2 / MCP1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:75.
Anti-CCL2 / MCP1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:75.

LSBio Ratings

IHC-plus™ CCL2 / MCP1 Antibody (aa62-89) for IHC, IF/Immunofluorescence, WB/Western, Flow LS-B10540 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)

Publications (1)

Conditional deletion of Ccl2 in smooth muscle cells does not reduce early atherosclerosis in mice. Stine Gunnersen, Jeong Tangkjær Shim, Fan Liu, Uwe J F Tietge, Charlotte Brandt Sørensen, Jacob Fog Bentzon. Atherosclerosis plus. 2024 March;55:12-20.

Customer Reviews (0)


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Requested From: United States
Date Requested: 11/24/2024