Human CASP3 / Caspase 3

Mouse

Human, Mouse, Rat (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

CAS3836

Unconjugated

Protein G purified

Unmodified

The large subunit domain of human Caspase-3.

Human CASP3 / Caspase 3

• Western blot (2 - 4 µg/ml)

Caspases, or cysteine-aspartic proteases, are a family of cysteine proteases that play essential roles in inflammation, apoptosis and necrosis. They are often referred to as the 'executioners of apoptosis.' Caspases are synthesized as inactive proenzymes comprising an N-terminal peptide (prodomain) together with one large and one small subunit. Active caspase-1 and -3 are heterodimers, containing two small and two large subunits. Active Caspase-3 subunits are 17kD (p17) and 12kD (p12). Pro Caspase-3 is inactive and has virtually no activity until it is cleaved by an initiator caspase. It is activated by Caspases -8, -9 and -10 ('initiator caspases') and then effects apoptosis by cleaving targeted cellular proteins. This antibody will detect the pro form and p17 and p12 active forms of Caspase-3.

1X PBS, pH 7.4, 0.1 mg/ml BSA, 0.05% Sodium Azide

Store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P42574 NCBI: NM_004346 NP_004337.2