Human CASP3 / Caspase 3

Mouse

Human, Mouse (tested or 100% immunogen sequence identity)

IgG Monoclonal

31A1067

Unconjugated

Protein G purified

Unmodified

Full length recombinant human Caspase-3 protein.

detects both pro Caspase-3 (~32 kD) and the large subunit of the active/cleaved form (~14-21 kD) of Caspase-3. The large subunit of the cleaved form may appear as one or two or even as a stack of bands depending on the presence or absence of the Caspase-3 pro-domain. It is highly recommended that a maximum sensitivity ECL substrate (Femto sensitive) be used for efficient detection of this in Western blot applications.

• IHC
• IHC - Paraffin (5 µg/ml)
• Western blot (2 - 3 µg/ml)

Immunohistochemistry: LS-B2125 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2125 was determined to be 5 ug/ml.

PBS, 0.05% Sodium Azide

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P42574 NCBI: NM_004346 NP_004337.2