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Catalog Number Size Price
LS-C209652-100 100 µg (1 mg/ml) $552 
CA2 / Carbonic Anhydrase II Antibody - Dot Blot of Rabbit Anti-Carbonic Anhydrase II Peroxidase Conjugated Antibody. Lane 1: 100ng. Lane 2: 33.3ng. Lane 3: 11.1ng. Lane 4: 3.7ng. Lane 5: 1.23ng. Secondary Antibody: Anti-Carbonic Anhydrase II HRP 1µg/mL.

Polyclonal Rabbit anti‑Bovine CA2 / Carbonic Anhydrase II Antibody (Lyophilized, HRP, IHC, WB) LS‑C209652

Polyclonal Rabbit anti‑Bovine CA2 / Carbonic Anhydrase II Antibody (Lyophilized, HRP, IHC, WB) LS‑C209652

Antibody:
CA2 / Carbonic Anhydrase II Rabbit anti-Bovine Polyclonal (Lyophilized) (HRP) Antibody
Application:
IHC, WB, ELISA, DB
Reactivity:
Bovine
Format:
Horseradish Peroxidase, Lyophilized
Price
Catalog Number
$552
LS-C209652-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
CA2 / Carbonic Anhydrase II Rabbit anti-Bovine Polyclonal (Lyophilized) (HRP) Antibody
Application:
IHC, WB, ELISA, DB
Reactivity:
Bovine
Format:
Horseradish Peroxidase, Lyophilized

Specifications

Description
Carbonic Anhydrase II antibody LS-C209652 is an HRP-conjugated rabbit polyclonal antibody to bovine Carbonic Anhydrase II (CA2). Validated for DB, ELISA, IHC and WB.
Target
Bovine CA2 / Carbonic Anhydrase II
Synonyms
CA2 | CA-II | CAII | CAC | Car2 | Carbonate dehydratase II | Carbonic anhydrase C | Carbonic anhydrase II | Carbonic dehydratase | Carbonic anhydrase 2 | Carbonic anhydrase B
Host
Rabbit
Reactivity
Bovine (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Horseradish Peroxidase
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Lyophilized
Immunogen
Carbonic Anhydrase II [Bovine Erythrocytes].
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Carbonic Anhydrase II [Bovine Erythrocytes]. Cross reactivity against Carbonic Anhydrase II from other tissues and species may occur but have not been specifically determined.
Applications
  • IHC (1:500 - 1:2500)
  • Western blot (1:1000 - 1:5000)
  • ELISA (1:5000 - 1:20000)
  • Dot Blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Anti-Carbonic anhydrase II has been assayed against 1.0 ug of Carbonic Anhydrase II [Bovine Erythrocytes] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:1000 to 1:4000 of the reconstitution concentration is suggested for this product. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CA2 / Carbonic Anhydrase II
P00918 NM_000067 NP_000058.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/26/2024