Human CA2 / Carbonic Anhydrase II

Rabbit

Human (tested or 100% immunogen sequence identity)

Polyclonal

Horseradish Peroxidase

Ion exchange chromatography

Unmodified

Carbonic Anhydrase II (Human Erythrocytes).

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti peroxidase, anti rabbit serum, as well as purified and partially purified carbonic anhydrase II (human erythrocytes).

• IHC (1:200 - 1:1000)
• IHC - Paraffin (5 µg/ml)
• Western blot (1:500 - 1:2000)
• ELISA (1:1000 - 1:6000)

Immunohistochemistry: LS-B2131 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2131 was determined to be 5 ug/ml. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

PBS, pH 7.2, 10 mg/ml BSA, 0.01% Gentamicin, 40% Glycerol

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P00918 NCBI: NM_000067 NP_000058.1