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Catalog Number Size Price
LS-C744964-100 100 µg (1 mg/ml) $567 
CA2 / Carbonic Anhydrase II Antibody - Immunohistochemistry with anti-carbonic anhydrase II antibody showing carbonic anhydrase II staining in nucleus and cytoplasm of proximal and distal tubules, bowman’s capsule and glomerular podocytes of human kidney (B) and in basal cells of retina of a mouse eye (C). Formalin fixed/paraffin embedded sections were subjected to heat induced epitope retrieval (HIER) at pH 6.2 and then incubated with rabbit anti-carbonic anhydrase II antibody at 4.0 µg/ml for 60 minutes. The reaction was developed using either MACH 1 universal HRP polymer detection (human kidney) or MACH 4 universal AP polymer detection system (mouse eye) and visualized with 3’3-diamino-benzidine substrate (DAB) or WARP RED.
CA2 / Carbonic Anhydrase II Antibody - Western Blot of rabbit Anti-Carbonic Anhydrase II primary antibody. Lane 1: Carbonic Anhydrase II. Lane 2: None. Load: 50 ng per lane. Primary antibody: Carbonic Anhydrase II primary antibody at 1:1,000 overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/Observed size: 29 kDa, 29 kDa for Carbonic Anhydrase II. Other band(s): None.
CA2 / Carbonic Anhydrase II Antibody - Immunohistochemistry with anti-carbonic anhydrase II antibody showing carbonic anhydrase II staining in nucleus and cytoplasm of proximal and distal tubules, bowman’s capsule and glomerular podocytes of human kidney (B) and in basal cells of retina of a mouse eye (C). Formalin fixed/paraffin embedded sections were subjected to heat induced epitope retrieval (HIER) at pH 6.2 and then incubated with rabbit anti-carbonic anhydrase II antibody at 4.0 µg/ml for 60 minutes. The reaction was developed using either MACH 1 universal HRP polymer detection (human kidney) or MACH 4 universal AP polymer detection system (mouse eye) and visualized with 3’3-diamino-benzidine substrate (DAB) or WARP RED.
CA2 / Carbonic Anhydrase II Antibody - Western Blot of rabbit Anti-Carbonic Anhydrase II primary antibody. Lane 1: Carbonic Anhydrase II. Lane 2: None. Load: 50 ng per lane. Primary antibody: Carbonic Anhydrase II primary antibody at 1:1,000 overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/Observed size: 29 kDa, 29 kDa for Carbonic Anhydrase II. Other band(s): None.
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Polyclonal Rabbit anti‑Human CA2 / Carbonic Anhydrase II Antibody (Lyophilized, IHC, WB) LS‑C744964

Polyclonal Rabbit anti‑Human CA2 / Carbonic Anhydrase II Antibody (Lyophilized, IHC, WB) LS‑C744964

Antibody:
CA2 / Carbonic Anhydrase II Rabbit anti-Human Polyclonal (Lyophilized) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Lyophilized
Price
Catalog Number
$567
LS-C744964-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
CA2 / Carbonic Anhydrase II Rabbit anti-Human Polyclonal (Lyophilized) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Lyophilized

Specifications

Description
Carbonic Anhydrase II antibody LS-C744964 is an unconjugated rabbit polyclonal antibody to human Carbonic Anhydrase II (CA2). Validated for ELISA, IHC and WB.
Target
Human CA2 / Carbonic Anhydrase II
Synonyms
CA2 | CA-II | CAII | CAC | Car2 | Carbonate dehydratase II | Carbonic anhydrase C | Carbonic anhydrase II | Carbonic dehydratase | Carbonic anhydrase 2 | Carbonic anhydrase B
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Lyophilized
Immunogen
Carbonic Anhydrase II Antibody was produced by repeated immunizations with human erythrocytes Carbonic Anhydrase II.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Carbonic Anhydrase II [Human Erythrocytes]. Cross reactivity against Carbonic Anhydrase II from other tissues and species may occur but have not been specifically determined.
Applications
  • IHC
  • Western blot (1:2000 - 1:10000)
  • ELISA (1:165000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CA2 / Carbonic Anhydrase II
P00918 NM_000067 NP_000058.1

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Requested From: United States
Date Requested: 12/26/2024