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Catalog Number	Size	Price
LS-C97187-400	400 µl	$393

BRAF / B-Raf Antibody - Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with BRAF Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

BRAF / B-Raf Antibody - Fluorescent image of C2C12 cell stained with BRAF Antibody. C2C12 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with BRAF primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). BRAF immunoreactivity is localized to Cytoplasm significantly.

BRAF / B-Raf Antibody - Western blot of BRAF Antibody polyclonal antibody(arrow). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BRAF gene (Lane 2) (Origene Technologies).

BRAF / B-Raf Antibody - B-RAF Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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Polyclonal Rabbit anti‑Human BRAF / B‑Raf Antibody (aa424‑453, IHC, IF, WB) LS‑C97187

Name: Anti-BRAF / B-Raf Antibody | Rabbit anti-Human Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C97187-400
Price: 393.0000 USD
Availability: InStock

Submit Review LSBio Guarantee

Antibody:

BRAF / B-Raf Rabbit anti-Human Polyclonal (aa424-453) Antibody

Application:

IHC, IHC-P, IF, WB, Flo

Reactivity:

Human

Format:

Unconjugated, Unmodified

$393

LS-C97187-400

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For Research Use Only

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Overview

Antibody:

BRAF / B-Raf Rabbit anti-Human Polyclonal (aa424-453) Antibody

Application:

IHC, IHC-P, IF, WB, Flo

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

B-Raf antibody LS-C97187 is an unconjugated rabbit polyclonal antibody to human B-Raf (BRAF) (aa424-453). Validated for Flow, IF, IHC and WB.

Target

Human BRAF / B-Raf

Synonyms

See All BRAF / B-Raf Antibodies

Host

Rabbit

Reactivity

Human (tested or 100% immunogen sequence identity)

Predicted

Mouse, Chicken (at least 90% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Ammonium sulfate precipitation

Modifications

Unmodified

Epitope

aa424-453

Specificity

This B-RAF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 424-453 amino acids from the Central region of human B-RAF.

Applications

IHC
IHC - Paraffin (1:10 - 1:50)
Immunofluorescence (1:10 - 1:50)
Western blot (1:1000)
Flow Cytometry (1:10 - 1:50)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

PBS, 0.09% Sodium Azide

Storage

Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About BRAF / B-Raf

Uniprot: P15056 NCBI: NM_004333 NP_004324.2