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| Catalog Number | Size | Price |
|---|---|---|
| LS-B3444-50 | 50 µl | $485 |
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IHC‑plus™ Monoclonal Mouse anti‑Human BRAF / B‑Raf Antibody (clone 1H12F1, IHC, WB) LS‑B3444
IHC‑plus™ Monoclonal Mouse anti‑Human BRAF / B‑Raf Antibody (clone 1H12F1, IHC, WB) LS‑B3444
Note: This antibody replaces LS-C41460, LS-C171370
Antibody:
BRAF / B-Raf Mouse anti-Human Monoclonal (1H12F1) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
BRAF / B-Raf Mouse anti-Human Monoclonal (1H12F1) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
B-Raf antibody LS-B3444 is an unconjugated mouse monoclonal antibody to human B-Raf (BRAF). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human BRAF / B-Raf
Synonyms
BRAF | 94 kDa B-raf protein | B-raf | BRAF1 | B Raf | Proto-oncogene B-Raf | p94 | B-RAF1 | NS7 | ONCOGENE BRAF | RAFB1
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Clone
1H12F1
Conjugations
Unconjugated
Purification
Ascites
Modifications
Unmodified
Immunogen
Purified recombinant fragment of BRAF expressed in E. coli.
Specificity
Ni-NTA purified truncated recombinant BRAF expressed in E. coli strain BL21 (DE3).
Applications
- IHC
- IHC - Paraffin (1:200)
- Western blot (1:100 - 1:500)
- ELISA (1:10000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B3444 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3444 was determined to be 1:200.
Presentation
Ascitic fluid, 0.03% sodium azide
Storage
Short term: store at 4°C. Long term: store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
About BRAF / B-Raf
Validation
Anti-BRAF antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-BRAF antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-BRAF antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-BRAF antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
See More About...
LSBio Ratings
IHC-plus™ BRAF / B-Raf Antibody (clone 1H12F1) for IHC, WB/Western, ELISA LS-B3444 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
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Featured Products
Species:
Human
Applications:
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Species:
Human, Mouse, Rat
Applications:
IHC, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human, Mouse, Rat, Chicken, Zebrafish
Applications:
IHC, IHC - Paraffin, Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/21/2024
Date Requested: 11/21/2024
