Human BMP4

Mouse

Human (tested or 100% immunogen sequence identity)

IgG2b Monoclonal

Unconjugated

Protein G purified

Unmodified

E.coli derived recombinant mature Human BMP-4 encoding amino acids 296-408 of P12644

Human BMP-4

• Western blot (0.25 - 0.5 µg/ml)
• ELISA (4 - 8 µg/ml)

Immunohistochemistry: This antibody stained HepG2 cells. The primary antibody was incubated at 3 ug/ml overnight at 4°C followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary. Information courtesy of the Cell Profiling group, SciLifeLab Stockholm. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human BMP-4 (5 ng/ml), a concentration of 0.4-0.6 ug/ml of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 ul/well), a concentration of 4-8 ug/ml of this antibody will detect at least 2000 pg/ml of recombinant human BMP-4 when used in conjunction with compatible secondary reagents. Western Blot: To detect hBMP-4 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBMP-4 is 2-4 ng/lane, under non-reducing conditions.

Lyophilized from PBS

Reconstitute in sterile water to 1 mg/ml.

Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P12644 NCBI: NM_001202 NP_001193.2