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Antibodies > BCL2 / Bcl-2 > LS-C413176
 
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Catalog Number Size Price
LS-C413176-100 100 µl (1 mg/ml) $341 
BCL2 / Bcl-2 Antibody - Immunohistochemical analysis of BCL2 (pT56) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
BCL2 / Bcl-2 Antibody - Immunofluorescent analysis of BCL2 (pT56) staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
BCL2 / Bcl-2 Antibody - Western blot analysis of BCL2 (pT56) expression in Jurkat (A); rat muscle (B) whole cell lysates.
BCL2 / Bcl-2 Antibody - Immunohistochemical analysis of BCL2 (pT56) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
BCL2 / Bcl-2 Antibody - Immunofluorescent analysis of BCL2 (pT56) staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
BCL2 / Bcl-2 Antibody - Western blot analysis of BCL2 (pT56) expression in Jurkat (A); rat muscle (B) whole cell lysates.
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Polyclonal Rabbit anti‑Human BCL2 / Bcl‑2 Antibody (phospho‑Thr56, IHC, IF, WB) LS‑C413176

Polyclonal Rabbit anti‑Human BCL2 / Bcl‑2 Antibody (phospho‑Thr56, IHC, IF, WB) LS‑C413176

Submit Review LSBio Guarantee
Antibody:
BCL2 / Bcl-2 Rabbit anti-Human Polyclonal (pThr56) Antibody
Application:
IHC, IHC-P, ICC, IF, WB, IP
Reactivity:
Human, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$341
LS-C413176-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
BCL2 / Bcl-2 Rabbit anti-Human Polyclonal (pThr56) Antibody
Application:
IHC, IHC-P, ICC, IF, WB, IP
Reactivity:
Human, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Bcl-2 antibody LS-C413176 is an unconjugated rabbit polyclonal antibody to Bcl-2 (BCL2) (pThr56) from human. It is reactive with human and rat. Validated for ICC, IF, IHC, IP and WB.
Target
Human BCL2 / Bcl-2
Synonyms
BCL2 | Apoptosis regulator Bcl-2 | B-cell CLL/lymphoma 2 | B-cell lymphoma protein 2 | Bcl-2 | PPP1R50
Host
Rabbit
Reactivity
Human, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the central region of human BCL2 (pT56).
Epitope
pThr56
Specificity
Recognizes endogenous levels of BCL2 (pT56) protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation (1:10 - 1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BCL2 / Bcl-2
P10415 NM_000633 NP_000624.2

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Featured Products

Product
BCL2 / Bcl‑2 Antibody (clone E17, Concentrated) LS‑C202809
Species: Human
Applications: IHC, IHC - Paraffin, IHC - Frozen
BCL2 / Bcl-2 Antibody - Anti-BCL2 / Bcl-2 antibody IHC of human kidney, tubules. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
IHC‑plus™ BCL2 / Bcl‑2 Antibody (aa46‑95) LS‑B6548
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
BCL2 / Bcl-2 Antibody - Formalin-fixed paraffin-embedded section from a secondary follicle of the mouse lymph node stained for Bcl-2 expression using Polyclonal Antibody to Bcl-2 at 1:2000. A, Abundant staining is seen in the mantle zone, and little staining is seen in the germinal center. A1 is a higher magnification of A.
BCL2 / Bcl‑2 Antibody (aa67‑86) LS‑C148229
Species: Mouse, Rat
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation
BCL2 / Bcl-2 Antibody - This image was taken for the unconjugated form of this product. Other forms have not been tested.
BCL2 / Bcl‑2 Antibody (aa41‑54, clone BL‑2, Magnetic beads) LS‑C171046
Species: Human
Applications: Immunoprecipitation
BCL2 / Bcl-2 Antibody - Western blot of Bcl-2 using Polyclonal Antibody to Bcl-2 at 1:2000.20 ug/protein was loaded per lane. Lane 1. Human Jurkat T cells. Lane 2. Human RS11 lymphoma cells. Lanes 3-6. Human breast cancer cases. Patient sample in lane 5 lacked detectible Bcl-2 expression.
BCL2 / Bcl‑2 Antibody (aa41‑54) LS‑C148227
Species: Human, Monkey, Dog, Pig
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation
BCL2 / Bcl-2 Antibody - Goat Anti-BCL2 Antibody (0.1µg/ml) staining of Human Lymph lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescencence.
BCL2 / Bcl‑2 Antibody (aa4‑18) LS‑C20131
Species: Human, Mouse, Rat, Dog
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay
See all BCL2 / Bcl-2 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/21/2024
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