Human BCL2 / Bcl-2

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1,k Recombinant Monoclonal

rBCL2/782

Unconjugated

Protein A/G purified

Azide-free, Carrier-free. Also available Unmodified.

Recombinant full-length human bcl-2 protein. Antigen Molecular Weight: 25-26 kDa

This antibody recognizes a protein of 25-26 kDa , identified as the bcl-2alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.

• IHC - Paraffin (1 - 2 µg/ml)
• Immunofluorescence (1 - 2 µg/ml)
• Western blot (1 - 2 µg/ml)
• Flow Cytometry (1 - 2 µg/10E6 cells)

Western Blotting (0.5-1 ug/ml) - Immunohistology (Formalin-fixed - 0.5-1 ug/ml for 30 minutes at RT - Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris with 1 mM EDTA Buffer, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.) Optimal dilution for a specific application should be determined.

10 mM PBS

Store at -20°C to -80°C for up to 2 years.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P10415 NCBI: NM_000633 NP_000624.2