Monoclonal Mouse anti‑Human BCL2 / Bcl‑2 Antibody (clone ARBC2‑1, Azide‑free,BSA‑free, IHC, IF, WB) LS‑C392072
Monoclonal Mouse anti‑Human BCL2 / Bcl‑2 Antibody (clone ARBC2‑1, Azide‑free,BSA‑free, IHC, IF, WB) LS‑C392072
Antibody:
BCL2 / Bcl-2 Mouse anti-Human Monoclonal (Azide-free, BSA-free) (ARBC2-1) Antibody
Application:
IHC, IHC-P, IF, WB, Flo
Format:
Unconjugated, Azide-free, BSA-free
Overview
Antibody:
BCL2 / Bcl-2 Mouse anti-Human Monoclonal (Azide-free, BSA-free) (ARBC2-1) Antibody
Application:
IHC, IHC-P, IF, WB, Flo
Format:
Unconjugated, Azide-free, BSA-free
Specifications
Description
Bcl-2 antibody LS-C392072 is an unconjugated mouse monoclonal antibody to human Bcl-2 (BCL2). Validated for Flow, IF, IHC and WB.
Target
Human BCL2 / Bcl-2
Synonyms
BCL2 | Apoptosis regulator Bcl-2 | B-cell CLL/lymphoma 2 | B-cell lymphoma protein 2 | Bcl-2 | PPP1R50
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Azide-free, BSA-free.
Also available
Unmodified or
Ready-to-Use.
Immunogen
Recombinant Full-length Human Protein.
Specificity
Human BCL2 / Bcl-2
Applications
- IHC
- IHC - Paraffin (1 - 2 µg/ml)
- Immunofluorescence (1 - 2 µg/ml)
- Western blot (1 - 2 µg/ml)
- Flow Cytometry (0.5 - 1 µg/10E6 cells)
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Usage
The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. Bcl2 suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release. [UniProt] Optimal dilution of the Bcl2 antibody should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.
Storage
Store at 2°C to 8°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human
Applications:
Immunoprecipitation
Species:
Mouse, Rat
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation
Species:
Human, Monkey, Dog, Pig
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation
Species:
Human, Mouse, Rat, Dog
Applications:
Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 10/19/2024