Human BCL2 / Bcl-2

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

100/D5

Unconjugated

Protein G purified

Unmodified. Also available Ready-to-Use, Azide-free, BSA-free.

Amino acids G aa PAPGIFSSQPG (41-54) of the human protein were used as the immunogen for this Bcl-2 antibody. Percent identity by BLAST analysis: Human, Chimpanzee, Cat, Pig, Dog (100%); Gorilla, Orangutan, Bovine, Goat (91%); Galago (82%).

Human BCL2 / Bcl-2

• IHC - Paraffin (0.5 - 1 µg/ml)
• Immunofluorescence (1 - 2 µg/ml)
• Western blot (0.5 - 1 µg/ml)
• Flow Cytometry (0.5 - 1 µg/10E6 cells)

This antibody recognizes a protein of 25-26kDa, identified as the Bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax. Expression of Bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of the protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, Bcl-2 antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express Bcl-2 and the small number in which the neoplastic cells are negative. ELISA: Order BSA-free format for coating.

1X PBS, 0.1 mg/ml BSA, 0.05% sodium azide

Store at 2°C to 8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P10415 NCBI: NM_000633 NP_000624.2