Human BCL2 / Bcl-2

Mouse

Human, Dog, Pig (tested or 100% immunogen sequence identity)

Mouse, Rat

IgG1,k Monoclonal

SPM530

Unconjugated

Protein G purified

Azide-free, BSA-free. Also available Unmodified.

Synthetic peptide (GAAPAPGIFSSQPG, aa41-54 of UniProt P10415) from the human protein. Percent identity by BLAST analysis: Human, Cat, Pig, Dog (100%); Bovine, Goat (93%); Horse (86%); Mouse, Rat (79%).

aa41-54

Human BCL2 / Bcl-2

• IHC
• IHC - Paraffin (0.5 - 1 µg/ml)
• Immunofluorescence (0.5 - 1 µg/ml)
• Western blot (0.5 - 1 µg/ml)
• Flow Cytometry (0.5 - 1 µg/10E6 cells)

The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. This antibody recognizes a protein of 25-26kDa, identified as the Bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative. The optimal dilution of the anti-Bcl-2 antibody for each application should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.

1X PBS

Store at 2°C to 8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P10415 NCBI: NM_000633 NP_000624.2