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Catalog Number Size Price
LS-C130334-250 250 µg $595 
Antibody

Monoclonal Mouse Basic Cytokeratin AE3 Antibody (clone AE‑3, IHC, WB) LS‑C130334

Monoclonal Mouse Basic Cytokeratin AE3 Antibody (clone AE‑3, IHC, WB) LS‑C130334

Antibody:
Basic Cytokeratin AE3 Mouse Monoclonal (AE-3) Antibody
Application:
IHC, IHC-P, IHC-Fr, WB, IP
Reactivity:
Mouse, Rat, Bovine, Rabbit, Chicken
Format:
Unconjugated, Unmodified
Price
Catalog Number
$595
LS-C130334-250
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
Basic Cytokeratin AE3 Mouse Monoclonal (AE-3) Antibody
Application:
IHC, IHC-P, IHC-Fr, WB, IP
Reactivity:
Mouse, Rat, Bovine, Rabbit, Chicken
Format:
Unconjugated, Unmodified

Specifications

Description
Basic Cytokeratin AE3 antibody LS-C130334 is an unconjugated mouse monoclonal antibody to Basic Cytokeratin AE3 from mouse, rat, bovine and other species. Validated for IHC, IP and WB.
Target
Basic Cytokeratin AE3
Host
Mouse
Reactivity
Mouse, Rat, Bovine, Rabbit, Chicken (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
AE-3
Conjugations
Unconjugated
Purification
Purified
Modifications
Unmodified
Immunogen
Keratin, Epithelial, Type II.
Specificity
Recognizes most of the basic (type II) keratins. Each epithelium contains at least one basic keratin. Broadly reactive with almost all epithelial and their neoplasms. Species cross-reactivity: Rabbit, mouse, rat, bovine, chicken.
Applications
  • IHC
  • IHC - Paraffin
  • IHC - Frozen
  • Western blot (1:100)
  • Immunoprecipitation
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Suitable for use in Western Blot and Immunohistochemistry. Western Blot: 1:100 Detects distinct MW's of 52, 54, 56, 58, 59, 64, and 65-67kD with all Ip's lower then 6.0. These MW's are believed to correspond with keratin 8, 7, 6, 5, 4, 3 and 1 & 2. Immunohistochemistry: 1:150-1:200 for use on formalin-fixed paraffin embedded tissues. Incubate for 30 minutes at 37°C. Can also be used with unfixed frozen sections.
Presentation
0.1% Sodium Azide
Storage
May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for up to 1 year.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/22/2024