Human Basic Cytokeratin AE3

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

AE-3

Unconjugated

Purified IgG

Concentrated

Human epidermal cells.

This antibody has shown to recognize most of the basic (type II) keratins. Each epithelium contains at least one basic keratin. In this way AE-3 is broadly reactive with almost all epithelial and there neoplasms. This antibody together with AE-1 has been used for studying the immuno-localization of epidermal keratins, tissue distribution and epithelia development. AE-1 and AE-3 have also been used extensively, individually or mixed, for distinguishing carcinoma from non-carcinoma. The antibody is cross-reactive with keratins of rabbit, mouse, rat, bovine and chicken. Within immunoblots AE-1 detects distinct MW's of 52 kD, 54 kD, 56 kD, 58 kD, 59 kD, 64 kD, and 65-67 kD with all Ip's lower then 6.0. These MW's are believed to correspond with respectively keratin 8, 7, 6, 5, 4, 3 and 1 & 2.

• IHC - Paraffin (1:100)
• IHC - Frozen
• Western blot (1:100)

In unfixed frozen sections and formalin-fixed paraffin embedded tissues. Also applicable in Western blotting. For use on formalin-fixed paraffin embedded tissues a dilution of approximately 1:150 to 1:200 is appropriate if section are incubated for 30 min. at 37°C. Possibly, higher dilutions can be used if sections are incubated at 4°C overnight. For use in Western blotting a dilution of 1:100 is advised.

0.01M PBS containing 0.1% Sodium azide and 1% BSA. Sourced in Ascites.

Store at 2-8°C.

For research use only. Intended for use by laboratory professionals.

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