Human B2M / Beta 2 Microglobulin

Mouse

Human (tested or 100% immunogen sequence identity)

IgG2b,k Monoclonal

B2M/961

Unconjugated

Protein G purified

Ready-to-Use. Also available Unmodified or Azide-free, BSA-free.

Full length recombinant human protein.

Human B2M / Beta 2 Microglobulin

• IHC
• IHC - Paraffin (1:1)
• Immunofluorescence
• Flow Cytometry

The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. Recognizes a protein of 12kDa, identified as beta-2 microglobulin. Major histocompatibility complex (MHC) class 1 molecules bind to antigens for presentation on the surface of cells. The proteasome is responsible for producing these antigens from the components of foreign pathogens. MHC class 1 molecules consist of an alpha heavy chain that contains three subdomains (alpha1, alpha2, alpha3) and a non-covalent associating light chain, known as beta-2-Microglobulin. Beta-2-Microglobulin associates with the alpha3 subdomain of the alpha heavy chain and forms an immunoglobulin domain-like structure that mediates proper folding and expression of MHC class 1 molecules. The alpha1 and alpha2 domains of the alpha heavy chain form the peptide antigen-binding cleft. Mutations in the beta-2-Microglobulin gene can enhance the progression of malignant melanoma phenotypes. Optimal dilution of the Beta-2 Microglobulin antibody should be determined by the researcher. Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

1X PBS, 0.1 mg/ml BSA, 0.05% sodium azide

Store at 2°C to 8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P61769 NCBI: NM_004048 NP_004039.1