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Catalog Number Size Price
LS-C819610-100 100 µl $412 
B2M / Beta 2 Microglobulin Antibody - Immunofluorescence staining of B2M in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-human B2M monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-mouse IgG secondary antibody (green). Positive staining was localized to Cytoplasm.
B2M / Beta 2 Microglobulin Antibody - Anti-B2M mouse monoclonal antibody at 1:500 dilution. Lane A: A-431 Whole Cell Lysate. Lane B: U-937 Whole Cell Lysate. Lane C: Raji Whole Cell Lysate. Lane D: HeLa Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 13 kDa. Observed band size: 13 kDa.
B2M / Beta 2 Microglobulin Antibody - Flow cytometric analysis of Human B2M / Beta-2-microglobulin expression on human whole blood lymphocytes. Cells were stained with purified anti-Human B2M / Beta-2-microglobulin, then a PE-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.
B2M / Beta 2 Microglobulin Antibody - Immunofluorescence staining of B2M in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-human B2M monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-mouse IgG secondary antibody (green). Positive staining was localized to Cytoplasm.
B2M / Beta 2 Microglobulin Antibody - Anti-B2M mouse monoclonal antibody at 1:500 dilution. Lane A: A-431 Whole Cell Lysate. Lane B: U-937 Whole Cell Lysate. Lane C: Raji Whole Cell Lysate. Lane D: HeLa Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 13 kDa. Observed band size: 13 kDa.
B2M / Beta 2 Microglobulin Antibody - Flow cytometric analysis of Human B2M / Beta-2-microglobulin expression on human whole blood lymphocytes. Cells were stained with purified anti-Human B2M / Beta-2-microglobulin, then a PE-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.
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Monoclonal Mouse anti‑Human B2M / Beta 2 Microglobulin Antibody (clone 35, Preservative Free, IF, WB) LS‑C819610

Monoclonal Mouse anti‑Human B2M / Beta 2 Microglobulin Antibody (clone 35, Preservative Free, IF, WB) LS‑C819610

Antibody:
B2M / Beta 2 Microglobulin Mouse anti-Human Monoclonal (Preservative Free) (35) Antibody
Application:
ICC, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Preservative Free
Price
Catalog Number
$412
LS-C819610-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
B2M / Beta 2 Microglobulin Mouse anti-Human Monoclonal (Preservative Free) (35) Antibody
Application:
ICC, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Preservative Free

Specifications

Description
Beta 2 Microglobulin antibody LS-C819610 is an unconjugated mouse monoclonal antibody to human Beta 2 Microglobulin (B2M). Validated for Flow, ICC, IF and WB.
Target
Human B2M / Beta 2 Microglobulin
Synonyms
B2M | Beta 2 Microglobulin | Beta-2-microglobulin | Beta-2-microglobin
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
35
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Preservative Free
Immunogen
Recombinant Human Beta-2 microglobulin/B2M Protein
Specificity
Human Beta-2 microglobulin
Applications
  • ICC (1:20 - 1:100)
  • Immunofluorescence (1:20 - 1:100)
  • Western blot (1:500 - 1:2000)
  • Flow Cytometry (1:25 - 1:100)
Usage
Applications should be user optimized.
Presentation
0.2 µm filtered solution in PBS
Storage
Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About B2M / Beta 2 Microglobulin
P61769 NM_004048 NP_004039.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/22/2024