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Antibodies > ATXN2 / SCA2 / Ataxin-2 > LS-C313216
 
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Catalog Number Size Price
LS-C313216-10 10 µg $318 
LS-C313216-100 100 µg (0.5 mg/ml) $470 
ATXN2 / SCA2 / Ataxin-2 Antibody - IHC analysis of ATX2 using anti-ATX2 antibody. ATX2 was detected in immunocytochemical section of A549 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml rabbit anti-ATX2 Antibody overnight at 4°C. DyLight®488 Conjugated Avidin was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ATXN2 / SCA2 / Ataxin-2 Antibody - IHC analysis of ATX2 using anti-ATX2 antibody. ATX2 was detected in immunocytochemical section of A549 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml rabbit anti-ATX2 Antibody overnight at 4°C. DyLight®488 Conjugated Avidin was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ATXN2 / SCA2 / Ataxin-2 Antibody - IHC analysis of ATX2 using anti-ATX2 antibody. ATX2 was detected in immunocytochemical section of A549 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml rabbit anti-ATX2 Antibody overnight at 4°C. DyLight®488 Conjugated Avidin was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ATXN2 / SCA2 / Ataxin-2 Antibody - WB of ATXN2 / SCA2 / Ataxin-2 antibody. Lane 1: PANC Cell Lysate. Lane 2: SMMC Cell Lysate. Lane 3: HELA Cell Lysate.
ATXN2 / SCA2 / Ataxin-2 Antibody - Flow Cytometry analysis of K562 cells using anti-ATX2 antibody. Overlay histogram showing K562 cells stained with anti-ATX2 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATX2 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
ATXN2 / SCA2 / Ataxin-2 Antibody - Flow Cytometry analysis of A549 cells using anti-ATX2 antibody. Overlay histogram showing A549 cells stained with anti-ATX2 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATX2 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
ATXN2 / SCA2 / Ataxin-2 Antibody - Flow Cytometry analysis of A431 cells using anti-ATX2 antibody. Overlay histogram showing A431 cells stained with anti-ATX2 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATX2 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
ATXN2 / SCA2 / Ataxin-2 Antibody - IHC analysis of ATX2 using anti-ATX2 antibody. ATX2 was detected in immunocytochemical section of A549 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml rabbit anti-ATX2 Antibody overnight at 4°C. DyLight®488 Conjugated Avidin was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ATXN2 / SCA2 / Ataxin-2 Antibody - IHC analysis of ATX2 using anti-ATX2 antibody. ATX2 was detected in immunocytochemical section of A549 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml rabbit anti-ATX2 Antibody overnight at 4°C. DyLight®488 Conjugated Avidin was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ATXN2 / SCA2 / Ataxin-2 Antibody - IHC analysis of ATX2 using anti-ATX2 antibody. ATX2 was detected in immunocytochemical section of A549 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml rabbit anti-ATX2 Antibody overnight at 4°C. DyLight®488 Conjugated Avidin was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ATXN2 / SCA2 / Ataxin-2 Antibody - WB of ATXN2 / SCA2 / Ataxin-2 antibody. Lane 1: PANC Cell Lysate. Lane 2: SMMC Cell Lysate. Lane 3: HELA Cell Lysate.
ATXN2 / SCA2 / Ataxin-2 Antibody - Flow Cytometry analysis of K562 cells using anti-ATX2 antibody. Overlay histogram showing K562 cells stained with anti-ATX2 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATX2 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
ATXN2 / SCA2 / Ataxin-2 Antibody - Flow Cytometry analysis of A549 cells using anti-ATX2 antibody. Overlay histogram showing A549 cells stained with anti-ATX2 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATX2 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
ATXN2 / SCA2 / Ataxin-2 Antibody - Flow Cytometry analysis of A431 cells using anti-ATX2 antibody. Overlay histogram showing A431 cells stained with anti-ATX2 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATX2 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Polyclonal Rabbit anti‑Human ATXN2 / SCA2 / Ataxin‑2 Antibody (aa1293‑1313, WB) LS‑C313216

Polyclonal Rabbit anti‑Human ATXN2 / SCA2 / Ataxin‑2 Antibody (aa1293‑1313, WB) LS‑C313216

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Note: This antibody replaces LS-C388038
Antibody:
ATXN2 / SCA2 / Ataxin-2 Rabbit anti-Human Polyclonal (aa1293-1313) Antibody
Application:
WB
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Guinea pig, Horse, Pig, Sheep
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C313216-10
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
ATXN2 / SCA2 / Ataxin-2 Rabbit anti-Human Polyclonal (aa1293-1313) Antibody
Application:
WB
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Guinea pig, Horse, Pig, Sheep
Format:
Unconjugated, Unmodified

Specifications

Description
Ataxin-2 antibody LS-C313216 is an unconjugated rabbit polyclonal antibody to Ataxin-2 (ATXN2 / SCA2) (aa1293-1313) from human. It is reactive with human, mouse, rat and other species. Validated for WB.
Target
Human ATXN2 / SCA2 / Ataxin-2
Synonyms
ATXN2 | ASL13 | Ataxin 2 | ATX2 | Ataxin-2 | SCA2 | TNRC13
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Guinea pig, Horse, Pig, Sheep (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the C-Terminus of human ATX2(1293-1313 aa TTAHFPYMTHPSVQAHHQQQL), identical to the related mouse sequence.
Epitope
aa1293-1313
Specificity
Expressed in the brain, heart, liver, skeletal muscle, pancreas and placenta. Isoform 1 is predominant in the brain and spinal cord. Isoform 4 is more abundant in the cerebellum. In the brain, broadly expressed in the amygdala, caudate nucleus, corpus callosum, hippocampus, hypothalamus, substantia nigra, subthalamic nucleus and thalamus. .
Applications
  • Western blot
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg Thimerosal, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ATXN2 / SCA2 / Ataxin-2
Q99700 NM_002973 NP_002964.3

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Featured Products

ATXN2 / SCA2 / Ataxin-2 Antibody - Western blot analysis of the lysates from HepG2 cells using ATXN2 antibody.
ATXN2 / SCA2 / Ataxin‑2 Antibody (aa725‑774) LS‑C200163
Species: Human
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay
ATXN2 / SCA2 / Ataxin-2 Antibody - Immunohistochemical analysis of Ataxin 2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
ATXN2 / SCA2 / Ataxin‑2 Antibody (Internal) LS‑C352811
Species: Human, Monkey
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation
ATXN2 / SCA2 / Ataxin-2 Antibody - Immunofluorescent analysis of HepG2 cells using ATXN2 Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
ATXN2 / SCA2 / Ataxin‑2 Antibody LS‑C501686
Species: Human
Applications: Immunofluorescence, Western blot, ELISA
ATXN2 / SCA2 / Ataxin-2 Antibody - Goat Anti-ATXN2 Antibody Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
ATXN2 / SCA2 / Ataxin‑2 Antibody (aa479‑489) LS‑C113029
Species: Human
Applications: IHC, Immunofluorescence, Peptide Enzyme-Linked Immunosorbent Assay
ATXN2 / SCA2 / Ataxin-2 Antibody - Western blot of Ataxin-2 antibody
ATXN2 / SCA2 / Ataxin‑2 Antibody (700‑780 aa, Internal) LS‑C382160
Species: Human
Applications: IHC, Western blot, ELISA
ATXN2 / SCA2 / Ataxin-2 Antibody - Detection of Human and Mouse ATX2/SCA2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, and mouse TCMK-1 cells. Antibodies: Affinity purified rabbit anti-ATX2/SCA2 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
ATXN2 / SCA2 / Ataxin‑2 Antibody (aa1262‑1312) LS‑C287945
Species: Human, Mouse
Applications: Western blot, Immunoprecipitation
See all ATXN2 / SCA2 / Ataxin-2 Antibodies

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/25/2024
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