Target
Human ATR
Synonyms
ATR | FRAP-related protein-1 | FCTCS | SCKL1 | MEC1 | Protein kinase ATR | Rad3 related protein | SCKL | FRAP-related protein 1 | FRP1 | XATR | Serine/threonine-protein kinase ATR | Ataxia telangiectasia and Rad3-related protein | mitosis entry checkpoint 1
Reactivity
Human, Monkey, Mouse, Rat, Dog, Xenopus, Fish
(tested or 100% immunogen sequence identity)
Predicted
Monkey, Mouse, Rat, Dog, Xenopus, Fish (at least 90% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Affinity purified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human ATR protein.
Specificity
This antibody is specific for human ATR protein. A BLAST analysis was used to suggest cross-reactivity with ATR from human, mouse, rat, monkey, dog, fish and Xenopus sources based on a 100% homology with the immunizing sequence. Cross-reactivity with ATR from other sources has not been determined.
Applications
- Western blot (1:1000 - 1:5000)
- ELISA (1:15000 - 1:70000)
Usage
This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 301 kD in size corresponding to ATR by western blotting in the appropriate cell lysate or extract. Lysates from HeLa, U2OS, RAW264.7 or HEK293 cells are suggested for western blotting. ATR is ubiquitously expressed, with highest expression levels in the testes. Note that isoform 2 is found in pancreas, placenta and liver but not in heart, testis and ovary.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee