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Catalog Number Size Price
LS-C773767-100 100 µg (1 mg/ml) $473 
ATG4A Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG4A Polyclonal Antibody. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG4A Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG4A Antibody (D) Composite.
ATG4A Antibody - Western blot analysis of Rat Pancreas cell lysates showing detection of 45.3 kDa ATG4A protein using Rabbit Anti-ATG4A Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Pancreas cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ATG4A Polyclonal Antibody  at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 45.3 kDa. Other Band(s): ~55-60 kDa.
ATG4A Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG4A Polyclonal Antibody. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG4A Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG4A Antibody (D) Composite.
ATG4A Antibody - Western blot analysis of Rat Pancreas cell lysates showing detection of 45.3 kDa ATG4A protein using Rabbit Anti-ATG4A Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Pancreas cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ATG4A Polyclonal Antibody  at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 45.3 kDa. Other Band(s): ~55-60 kDa.
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Polyclonal Rabbit anti‑Human ATG4A Antibody (FITC, IF, WB) LS‑C773767

Polyclonal Rabbit anti‑Human ATG4A Antibody (FITC, IF, WB) LS‑C773767

Antibody:
ATG4A Rabbit anti-Human Polyclonal (FITC) Antibody
Application:
IF, WB
Reactivity:
Human, Mouse, Rat
Format:
FITC, Unmodified
Other formats:
Price
Catalog Number
$473
LS-C773767-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
ATG4A Rabbit anti-Human Polyclonal (FITC) Antibody
Application:
IF, WB
Reactivity:
Human, Mouse, Rat
Format:
FITC, Unmodified
Other formats:

Specifications

Description
ATG4A antibody LS-C773767 is an FITC-conjugated rabbit polyclonal antibody to ATG4A from human. It is reactive with human, mouse and rat. Validated for IF and WB.
Target
Human ATG4A
Synonyms
ATG4A | AUTL2 | Autophagin-2 | APG4A | Autophagin 2 | Cysteine protease ATG4A | HAPG4A | APG4 autophagy 4 homolog A
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
FITC. Also available conjugated with Atto 390, Atto 488, Atto 594, Biotin, PerCP, RPE.
Purification
Peptide Affinity Purified
Modifications
Unmodified
Immunogen
Synthetic peptide from the mid-protein of Human ATG4A (aa. 102-113)
Specificity
Detects ~45 kDa.
Applications
  • Immunofluorescence (1:100)
  • Western blot (1:1000)
  • Applications tested for the base form of this product only
Usage
Applications should be user optimized.
Presentation
PBS, 0.09% Sodium Azide, 50% Glycerol
Storage
Store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ATG4A
Q8WYN0 NM_052936 Q8WYN0

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/25/2024