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ATG4A

autophagy related 4A, cysteine peptidase

Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Has also an activity of delipidating enzyme for the PE-conjugated forms.

Gene Name: autophagy related 4A, cysteine peptidase
Synonyms: ATG4A, AUTL2, Autophagin-2, APG4A, Autophagin 2, Cysteine protease ATG4A, HAPG4A, APG4 autophagy 4 homolog A
Target Sequences: NM_052936 Q8WYN0 Q8WYN0

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Products
Antibodies (49)
Primary (49)
ATG4A (49)
Human (49)
Mouse (25)
Rat (15)
Bat (3)
Bovine (3)
Chimpanzee (3)
Dog (3)
Gibbon (6)
Hamster (3)
Horse (3)
Monkey (3)
Orangutan (6)
Pig (3)
Rabbit (3)
Zebrafish (3)
IHC (10)
IHC-P (4)
WB (46)
Flo (2)
ELISA (13)
ICC (2)
IF (21)
IP (5)
Peptide-ELISA (3)
rabbit (44)
mouse (4)
sheep (1)
Product Group
IHCPlus (1)
IgG (15)
IgG1 (1)
IgG2b (2)
IgG2b,k (1)
monoclonal mc (4)
polyclonal pc (45)
1458CT808.66.25.69 (1)
8C9C2 (1)
8E8G2 (1)
APC Conjugated (1)
Atto 390 Conjugated (2)
Atto 488 Conjugated (2)
Atto 594 Conjugated (2)
Biotin Conjugated (5)
FITC Conjugated (5)
HRP Conjugated (3)
PerCP Conjugated (2)
RPE Conjugated (2)
Unconjugated (25)
aa1-214 (4)
aa251-300 (4)
aa91-140 (4)
aa2-398 (2)
50-130 aa, Internal (1)
aa1-30 (1)
aa100-114 (1)
aa363-392 (1)
aa81-130 (1)
No (49)
ATG4A Antibody - Immunofluorescence analysis of Hela cells using ATG4A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
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ATG4A Mouse anti-Human Monoclonal (8C9C2) Antibody
Human
ELISA, Flo, ICC, WB
Unconjugated
100 µg/$459
ATG4A Antibody - Immunohistochemical analysis of ATG4A staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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ATG4A Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
ICC, IF, IHC, WB
Unconjugated
30 µl/$255; 100 µl/$299; 200 µl/$351
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ATG4A Rabbit anti-Human Polyclonal (50-130 aa, Internal) Antibody
Mouse, Human
ELISA, IF, IHC, WB
Unconjugated
50 µg/$295; 100 µg/$335; 200 µg/$394
ATG4A Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
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ATG4A Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
ELISA, IHC, IHC-P, WB
Unconjugated
0.05 mg/$460
ATG4A Antibody - Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using ATG4A Antibody. The picture on the right is blocked with the synthesized peptide.
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ATG4A Rabbit anti-Human Polyclonal (aa81-130) Antibody
Mouse, Human
IF, IHC, IHC-P, Peptide-ELISA
Unconjugated
50 µl/$334; 100 µl/$397
ATG4A Antibody - Western blot analysis using ATG4A mAb against HEK293 (1) and ATG4A (AA: 258-398)-hIgGFc transfected HEK293 (2) cell lysate.
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ATG4A Mouse anti-Human Monoclonal (8E8G2) Antibody
Human
ELISA, Flo, WB
Unconjugated
100 µg/$459
ATG4A Antibody - Peptide - + Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using ATG4A antibody.
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ATG4A Rabbit anti-Human Polyclonal Antibody
Mouse, Human
ELISA, IF, IHC
Unconjugated
100 µg/$357
ATG4A Antibody - Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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ATG4A Rabbit anti-Human Polyclonal (aa363-392) Antibody
Mouse, Human
IHC, IHC-P, WB
Unconjugated
400 µl/$393
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ATG4A Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IHC, WB
Unconjugated
20 µl/$254; 60 µl/$296; 120 µl/$355; 200 µl/$450
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ATG4A Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IHC, IP, WB
Unconjugated
50 µl/$356; 100 µl/$461
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ATG4A Mouse anti-Human Monoclonal (aa2-398) Antibody
Human
ELISA, IP, WB
Unconjugated
100 µg/$884
ATG4A Antibody - Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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ATG4A Rabbit anti-Human Polyclonal (aa1-30) Antibody
Mouse, Human
IHC, IHC-P, WB
Unconjugated
400 µl/$393
ATG4A Antibody - Western blot analysis of extracts of various cell lines.
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ATG4A Rabbit anti-Human Polyclonal Antibody
Mouse, Human
IHC, IP, WB
Unconjugated
20 µl/$275; 100 µl/$389
ATG4A Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG4A Polyclonal Antibody. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG4A Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG4A Antibody (D) Composite.
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ATG4A Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IF, WB
Unconjugated
100 µg/$442
ATG4A Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG4A Polyclonal Antibody. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG4A Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG4A Antibody (D) Composite.
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ATG4A Rabbit anti-Human Polyclonal Antibody
Human
IF, WB
Unconjugated
100 µg/$442
ATG4A Antibody - Western blot analysis of mouse-liver lysate, antibody was diluted at 1000. Secondary antibody was diluted at 1:20000.
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ATG4A Rabbit anti-Human Polyclonal Antibody
Rat, Human
ELISA, WB
Unconjugated
50 µg/$295; 100 µg/$335; 200 µg/$394
ATG4A Antibody - Western blot of lysate from K562 cell line, using ATG4A antibody diluted at 1:500. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20 ug.
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ATG4A Mouse anti-Human Monoclonal (1458CT808.66.25.69) Antibody
Human
Peptide-ELISA, WB
Unconjugated
400 µl/$393
ATG4A Antibody - Western blot. All lanes: ATG4A antibody at 2 ug/ml+mouse brain tissue. Secondary antibody: Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 45 kDa. Observed band size: 45 kDa.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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ATG4A Rabbit anti-Human Polyclonal (aa1-214) Antibody
Mouse, Human
ELISA, WB
Unconjugated
50 µg/$294; 100 µg/$360
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ATG4A Sheep anti-Human Polyclonal (aa2-398) Antibody
Human
ELISA, WB
Unconjugated
100 µg/$930
ATG4A Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG4A Polyclonal Antibody. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG4A Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG4A Antibody (D) Composite.
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ATG4A Rabbit anti-Human Polyclonal (FITC) Antibody
Human
IF, WB
FITC Conjugated
100 µg/$473
ATG4A Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG4A Polyclonal Antibody. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG4A Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG4A Antibody (D) Composite.
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ATG4A Rabbit anti-Human Polyclonal (Atto 594) Antibody
Human
IF, WB
Atto 594 Conjugated
100 µg/$479
ATG4A Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG4A Polyclonal Antibody. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG4A Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG4A Antibody (D) Composite.
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ATG4A Rabbit anti-Human Polyclonal (Biotin) Antibody
Mouse, Rat, Human
IF, WB
Biotin Conjugated
100 µg/$477
ATG4A Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG4A Polyclonal Antibody. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG4A Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG4A Antibody (D) Composite.
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ATG4A Rabbit anti-Human Polyclonal (FITC) Antibody
Mouse, Rat, Human
IF, WB
FITC Conjugated
100 µg/$473
ATG4A Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG4A Polyclonal Antibody. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG4A Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG4A Antibody (D) Composite.
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ATG4A Rabbit anti-Human Polyclonal (Atto 594) Antibody
Mouse, Rat, Human
IF, WB
Atto 594 Conjugated
100 µg/$479
ATG4A Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG4A Polyclonal Antibody. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG4A Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG4A Antibody (D) Composite.
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ATG4A Rabbit anti-Human Polyclonal (PerCP) Antibody
Mouse, Rat, Human
IF, WB
PerCP Conjugated
100 µg/$479
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