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Catalog Number	Size	Price
LS-C407695-10	10 µg	$318
LS-C407695-100	100 µg	$470

ATG14 Antibody - ATG14L antibody IHC-paraffin. IHC(P): Human Lung Cancer Tissue.

ATG14 Antibody - ATG14L antibody IHC-paraffin. IHC(P): Rat Spleen Tissue.

ATG14 Antibody - ATG14L antibody Western blot. All lanes: Anti ATG14L at 0.5 ug/ml. Lane 1: Rat Brain Tissue Lysate at 50 ug. Lane 2: HELA Whole Cell Lysate at 40 ug. Predicted band size: 59 kD. Observed band size: 59 kD.

ATG14 Antibody - Flow Cytometry analysis of PC-3 cells using anti-ATG14L antibody. Overlay histogram showing PC-3 cells stained with anti-ATG14L antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATG14L Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

ATG14 Antibody - Flow Cytometry analysis of A431 cells using anti-ATG14L antibody. Overlay histogram showing A431 cells stained with anti-ATG14L antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATG14L Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

ATG14 Antibody - Flow Cytometry analysis of SiHa cells using anti-ATG14L antibody. Overlay histogram showing SiHa cells stained with anti-ATG14L antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATG14L Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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Polyclonal Rabbit anti‑Human ATG14 Antibody (aa70‑101, IHC, WB) LS‑C407695

Name: Anti-ATG14 Antibody | Rabbit anti-Human Polyclonal IHC,WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C407695-10
Price: 318.0000 USD
Availability: InStock

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Antibody:

ATG14 Rabbit anti-Human Polyclonal (aa70-101) Antibody

Application:

IHC, IHC-P, WB

Reactivity:

Human, Rat

Format:

Unconjugated, Unmodified

$318

LS-C407695-10

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

ATG14 Rabbit anti-Human Polyclonal (aa70-101) Antibody

Application:

IHC, IHC-P, WB

Reactivity:

Human, Rat

Format:

Unconjugated, Unmodified

Specifications

Description

ATG14 antibody LS-C407695 is an unconjugated rabbit polyclonal antibody to ATG14 (aa70-101) from human. It is reactive with human and rat. Validated for IHC and WB.

Target

Human ATG14

Synonyms

See All ATG14 Antibodies

Host

Rabbit

Reactivity

Human, Rat (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunogen affinity purified

Modifications

Unmodified

Immunogen

A synthetic peptide corresponding to a sequence at the N-Terminus of human ATG14L (70-101 aa RDRERFIDKKERLSRLKSKQEEFQKEVLKAME), different from the related mouse and rat sequences by two amino acids.

Epitope

aa70-101

Applications

IHC
IHC - Paraffin (0.5 - 1 µg/ml)
Western blot (0.1 - 0.5 µg/ml)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

IHC: Antigen retrieval by boiling the paraffin sections in 10 mM citrate buffer, pH6.0, for 20 minutes is required for the staining of formalin/paraffin sections.

Presentation

Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About ATG14

Uniprot: Q6ZNE5 NCBI: AB020638 BAA74854.2