Target
Mouse ARHGEF7
Synonyms
ARHGEF7 | COOL1 | COOL-1 | Hypothetical protein kiaa0142 | Nbla10314 | p85 | p85COOL1 | p85SPR | PIXB | KIAA0142 | p50 | PAK3BP | BETA-PIX | p50BP
Reactivity
Mouse, Human, Rat
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Protein A purified
Immunogen
Recombinant beta -Pix SH3 domain.
Specificity
Recognizes the multiple splice variants of human, mouse and rat beta -Pix a, b, b
Applications
- ICC (1:1000)
- Western blot (1:2000 - 1:5000)
- ELISA (1:5000 - 1:10000)
Usage
ELISA - Direct and indirect: 1:5000-1:10000. Immunocytochemistry - 1:1000. Western Blot - 1:2000-1:5000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature.
Presentation
PBS, pH 7.4, no preservatives.
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee