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Catalog Number Size Price
LS-C744614-25 25 µl (70 mg/ml) $304 
ARFGAP3 Antibody - Immunofluorescence Microscopy of rabbit Anti-ArfGAP3 Antibody. Tissue: HeLa Whole Cell. Fixation: MeOH. Antigen retrieval: not required. Primary antibody: ArfGAP3 antibody at 1:100 for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: ArfGAP3 is cytoplasmic. Staining: ArfGAP3 as green fluorescent sinal.
ARFGAP3 Antibody - Western Blot of rabbit Anti-ArfGAP3 Antibody. Lane 1 (C): HeLa Whole Cell. Lane 2 (si): HeLa Whole Cell siRNA treated. Load: 10 µg per lane. Primary antibody: ArfGAP3 antibody at 1:1000 for overnight at 4°C. Secondary antibody: rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 57 kDa for endogenous Arf-GAP3. Other band(s): non-specific band ~50kDa.
ARFGAP3 Antibody - Immunofluorescence Microscopy of rabbit Anti-ArfGAP3 Antibody. Tissue: HeLa Whole Cell. Fixation: MeOH. Antigen retrieval: not required. Primary antibody: ArfGAP3 antibody at 1:100 for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: ArfGAP3 is cytoplasmic. Staining: ArfGAP3 as green fluorescent sinal.
ARFGAP3 Antibody - Western Blot of rabbit Anti-ArfGAP3 Antibody. Lane 1 (C): HeLa Whole Cell. Lane 2 (si): HeLa Whole Cell siRNA treated. Load: 10 µg per lane. Primary antibody: ArfGAP3 antibody at 1:1000 for overnight at 4°C. Secondary antibody: rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 57 kDa for endogenous Arf-GAP3. Other band(s): non-specific band ~50kDa.
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Polyclonal Rabbit anti‑Human ARFGAP3 Antibody (IF, WB) LS‑C744614

Polyclonal Rabbit anti‑Human ARFGAP3 Antibody (IF, WB) LS‑C744614

Antibody:
ARFGAP3 Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$304
LS-C744614-25
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
ARFGAP3 Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
ARFGAP3 antibody LS-C744614 is an unconjugated rabbit polyclonal antibody to human ARFGAP3. Validated for ELISA, IF, IP and WB.
Target
Human ARFGAP3
Synonyms
ARFGAP3 | ARF GAP 3
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Delipidated and defibrinated
Modifications
Unmodified
Immunogen
This whole rabbit serum was prepared by repeated immunizations with a truncated recombinant sequence of ArfGAP3 fused to GST.
Specificity
The antibody detects ArfGAP3 in cell lysates. A BLAST analysis was used to suggest cross reactivity with human, monkey, and orangutan for ArfGAP3. Cross-reactivity with AfGAP3 from other sources have not been determined.
Applications
  • Immunofluorescence (1:100)
  • Western blot (1:1000)
  • Immunoprecipitation
  • ELISA (1:5000)
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ARFGAP3
Q9NP61 NM_014570 NP_055385.3

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/22/2024