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Catalog Number Size Price
LS-C209178-100 100 µl (70 mg/ml) $567 
ARFGAP3 Antibody - Immunofluorescence Microscopy of Rabbit Anti-ArfGAP3 Antibody. Tissue: HeLa Whole Cell. Fixation: MeOH. Antigen retrieval: not required. Primary antibody: ArfGAP3 antibody at 1:100 for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: ArfGAP3 is cytoplasmic. Staining: ArfGAP3 as green fluorescent signal.
ARFGAP3 Antibody - Western Blot of Rabbit Anti-ArfGAP3 Antibody. Lane 1 (C): HeLa Whole Cell. Lane 2 (si): HeLa Whole Cell siRNA treated. Load: 10 ug per lane. Primary antibody: ArfGAP3 antibody at 1:1000 for overnight at 4 degrees C. Secondary antibody: IRDye800 alpha rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4 degrees C. Predicted/Observed size: 57 kDa for endogenous Arf-GAP3. Other band(s): non-specific band ~50kDa.
ARFGAP3 Antibody - Immunofluorescence Microscopy of Rabbit Anti-ArfGAP3 Antibody. Tissue: HeLa Whole Cell. Fixation: MeOH. Antigen retrieval: not required. Primary antibody: ArfGAP3 antibody at 1:100 for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: ArfGAP3 is cytoplasmic. Staining: ArfGAP3 as green fluorescent signal.
ARFGAP3 Antibody - Western Blot of Rabbit Anti-ArfGAP3 Antibody. Lane 1 (C): HeLa Whole Cell. Lane 2 (si): HeLa Whole Cell siRNA treated. Load: 10 ug per lane. Primary antibody: ArfGAP3 antibody at 1:1000 for overnight at 4 degrees C. Secondary antibody: IRDye800 alpha rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4 degrees C. Predicted/Observed size: 57 kDa for endogenous Arf-GAP3. Other band(s): non-specific band ~50kDa.
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Polyclonal Rabbit anti‑Human ARFGAP3 Antibody (IF, WB) LS‑C209178

Polyclonal Rabbit anti‑Human ARFGAP3 Antibody (IF, WB) LS‑C209178

Antibody:
ARFGAP3 Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$567
LS-C209178-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
ARFGAP3 Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
ARFGAP3 antibody LS-C209178 is an unconjugated rabbit polyclonal antibody to human ARFGAP3. Validated for ELISA, IF, IP and WB.
Target
Human ARFGAP3
Synonyms
ARFGAP3 | ARF GAP 3
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Delipidated and defibrinated
Modifications
Unmodified
Immunogen
This whole rabbit serum was prepared by repeated immunizations with a truncated recombinant sequence of ArfGAP3 fused to GST.
Specificity
The antibody detects ArfGAP3 in cell lysates. A BLAST analysis was used to suggest cross reactivity with human, monkey, and orangutan for ArfGAP3. Cross-reactivity with AfGAP3 from other sources have not been determined.
Applications
  • Immunofluorescence (1:100)
  • Western blot (1:1000)
  • Immunoprecipitation
  • ELISA (1:5000)
Usage
ArfGAP3 has been tested for use in ELISA, Immunofluorescence, and western blotting. Expect a band approximately 57 kD in size by western blotting in the appropriate cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ARFGAP3
Q9NP61 NM_014570 NP_055385.3

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 11/22/2024