Human AR / Androgen Receptor

Mouse

Human (tested or 100% immunogen sequence identity)

Mouse

IgG Monoclonal

AR441 + DHTR/882

Unconjugated

Protein G purified

Ready-to-Use. Also available Unmodified or Azide-free, BSA-free.

Synthetic peptide (STEDTAEYSPFKGGYTK, aa300-316 of UniProt P10275) of human AR (AR441) and recombinant human AR protein (DHTR/882). Percent identity by BLAST analysis: Human (100%); Mouse, Sheep, Bovine, Pig (82%).

aa300-316

Human AR / Androgen receptor

• IHC
• IHC - Paraffin (1:1)
• Immunofluorescence

The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. Recognizes a protein of 110kDa, which is identified as androgen receptor (AR). It reacts with full length, and the newly described A form of the receptor. It does not cross react with estrogen, progesterone, or glucocorticoid receptors. The expression of AR is reportedly inversely correlated with histologic grade i.e. well differentiated prostate tumors show higher expression than the poorly differentiated tumors. In prostate cancer, AR has been proposed, as a marker of hormone-responsiveness and thus it may be useful in identifying patients likely to benefit from anti-androgen therapy. Anti-androgen receptor has been useful clinically in differentiating morpheaform basal cell carcinoma (mBCC) from desmoplastic trichoepithelioma (DTE) in the skin. This mAb is superb for staining of formalin/paraffin tissues. Optimal dilution of the Androgen Receptor antibody cocktail should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

1X PBS, 0.1 mg/ml BSA, 0.05% sodium azide

Store at 2°C to 8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P10275 NCBI: NM_000044 NP_000035.2