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Antibodies > APP / Beta Amyloid Precursor > LS-C343960
 
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Catalog Number Size Price
LS-C343960-10 10 µg $318 
LS-C343960-100 100 µg (0.5 mg/ml) $470 
APP / Beta Amyloid Precursor Antibody - IHC analysis of APP using anti-APP antibody. APP was detected in paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-APP Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
APP / Beta Amyloid Precursor Antibody - IHC analysis of APP using anti-APP antibody. APP was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-APP Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
APP / Beta Amyloid Precursor Antibody - IHC analysis of APP using anti-APP antibody. APP was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-APP Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
APP / Beta Amyloid Precursor Antibody - IHC analysis of APP using anti-APP antibody. APP was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-APP Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
APP / Beta Amyloid Precursor Antibody - beta Amyloid antibody IHC-paraffin: Rat Brain Tissue.
APP / Beta Amyloid Precursor Antibody - beta Amyloid antibody IHC-paraffin: Mouse Brain Tissue.
APP / Beta Amyloid Precursor Antibody - IHC analysis of APP using anti-APP antibody. APP was detected in paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-APP Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
APP / Beta Amyloid Precursor Antibody - IF analysis of APP using anti-APP antibody APP was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-APP Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
APP / Beta Amyloid Precursor Antibody - Western blot analysis of APP using anti-APP antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates,Lane 2: human U-87MG whole cell lysates,Lane 3: human T-47D whole cell lysates,Lane 4: human A549 whole cell lysates,Lane 5: human U2OS whole cell lysates,Lane 6: rat brain tissue lysates,Lane 7: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APP antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for APP at approximately 120KD. The expected band size for APP is at 87KD.
APP / Beta Amyloid Precursor Antibody - beta Amyloid antibody Western blot. All lanes: Anti beta Amyloid at 0.5 ug/ml. Lane 1: Rat Brain Tissue Lysate at 50 ug. Lane 2: Mouse Brain Tissue Lysate at 50 ug. Predicted band size: 87 kD. Observed band size: 87 kD.
APP / Beta Amyloid Precursor Antibody - beta Amyloid antibody Western blot. All lanes: Anti beta Amyloid at 0.5 ug/ml. WB: Recombinant Human beta-Amyloid Protein 0.5ng. Predicted band size: 29 kD. Observed band size: 29 kD.
APP / Beta Amyloid Precursor Antibody - IHC analysis of APP using anti-APP antibody. APP was detected in paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-APP Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
APP / Beta Amyloid Precursor Antibody - IHC analysis of APP using anti-APP antibody. APP was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-APP Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
APP / Beta Amyloid Precursor Antibody - IHC analysis of APP using anti-APP antibody. APP was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-APP Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
APP / Beta Amyloid Precursor Antibody - IHC analysis of APP using anti-APP antibody. APP was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-APP Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
APP / Beta Amyloid Precursor Antibody - beta Amyloid antibody IHC-paraffin: Rat Brain Tissue.
APP / Beta Amyloid Precursor Antibody - beta Amyloid antibody IHC-paraffin: Mouse Brain Tissue.
APP / Beta Amyloid Precursor Antibody - IHC analysis of APP using anti-APP antibody. APP was detected in paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-APP Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
APP / Beta Amyloid Precursor Antibody - IF analysis of APP using anti-APP antibody APP was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-APP Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
APP / Beta Amyloid Precursor Antibody - Western blot analysis of APP using anti-APP antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates,Lane 2: human U-87MG whole cell lysates,Lane 3: human T-47D whole cell lysates,Lane 4: human A549 whole cell lysates,Lane 5: human U2OS whole cell lysates,Lane 6: rat brain tissue lysates,Lane 7: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APP antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for APP at approximately 120KD. The expected band size for APP is at 87KD.
APP / Beta Amyloid Precursor Antibody - beta Amyloid antibody Western blot. All lanes: Anti beta Amyloid at 0.5 ug/ml. Lane 1: Rat Brain Tissue Lysate at 50 ug. Lane 2: Mouse Brain Tissue Lysate at 50 ug. Predicted band size: 87 kD. Observed band size: 87 kD.
APP / Beta Amyloid Precursor Antibody - beta Amyloid antibody Western blot. All lanes: Anti beta Amyloid at 0.5 ug/ml. WB: Recombinant Human beta-Amyloid Protein 0.5ng. Predicted band size: 29 kD. Observed band size: 29 kD.
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Polyclonal Rabbit anti‑Human APP / Beta Amyloid Precursor Antibody (aa672‑713, IHC, WB) LS‑C343960

Polyclonal Rabbit anti‑Human APP / Beta Amyloid Precursor Antibody (aa672‑713, IHC, WB) LS‑C343960

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Antibody:
APP / Beta Amyloid Precursor Rabbit anti-Human Polyclonal (aa672-713) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C343960-10
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
APP / Beta Amyloid Precursor Rabbit anti-Human Polyclonal (aa672-713) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Beta Amyloid Precursor antibody LS-C343960 is an unconjugated rabbit polyclonal antibody to Beta Amyloid Precursor (APP) (aa672-713) from human. It is reactive with human, mouse and rat. Validated for IHC and WB.
Target
Human APP / Beta Amyloid Precursor
Synonyms
APP | ABPP | A4 | ABETA | AD1 | Alzheimer disease | Amyloid beta | Beta-amyloid peptide | APPI | Beta amyloid | CVAP | AAA | PN2 | PreA4 | Amyloid beta A4 protein | Peptidase nexin-II | CTFgamma | PN-II | Protease nexin-II
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the C-Terminus of human APP (672-713 aa DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA), different from the related mouse and rat sequences by three amino acids.
Epitope
aa672-713
Specificity
Expressed in all fetal tissues examined with highest levels in brain, kidney, heart and spleen. Weak expression in liver. In adult brain, highest expression found in the frontal lobe of the cortex and in the anterior perisylvian cortex- opercular gyri. Moderate expression in the cerebellar cortex, the posterior perisylvian cortex-opercular gyri and the temporal associated cortex. Weak expression found in the striate, extra- striate and motor cortices. Expressed in cerebrospinal fluid, and plasma. Isoform APP695 is the predominant form in neuronal tissue, isoform APP751 and isoform APP770 are widely expressed in non- neuronal cells. Isoform APP751 is the most abundant form in T- lymphocytes. Appican is expressed in astrocytes. .
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About APP / Beta Amyloid Precursor
P05067 NM_000484 NP_000475.1

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Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/29/2024
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